Table 1.

Abundance of the NS5A protein in Replicon 1b cells following treatment with HCV inhibitorsa

Time (h)Mean % NS5A protein level relative to control ± SD
DMSOHCV-796BILN-2061NS5Ai 3NS5Ai 4
1010096.5 ± 1.595 ± 2100.5 ± 2.592.5 ± 1.5
2410091.5 ± 0.594.5 ± 0.597 ± 197.5 ± 0.5
4810084.5 ± 1.575.5 ± 1.574 ± 274 ± 2
7210087 ± 274 ± 273 ± 265.5 ± 1.5
  • a Densitometry was used to analyze the relative levels of the NS5A protein revealed following Western blot analysis of cell extracts prepared at different times after exposure to the inhibitor at 10× the IC50. Data were normalized to DMSO values for each time point and are expressed as averages of data from 2 experiments ± standard errors of the means (data in Fig. 5C contributed 1 set of data; Western blot data for 2 experiments not shown).