Table 1.

Numbers of unenveloped, partially enveloped, and fully enveloped particles in Vero and VD60/gB47 cells infected with F-BAC gB/gDa

Cell type and virusNo. (%) of:Total
Unenveloped cytoplasmic capsidsPartially enveloped cytoplasmic capsidsEnveloped cytoplasmic particlesEnveloped cell surface particles
Vero cells
    F-BAC (w.t.)140 (11)3 (0.2)191 (15)939 (74)1,273
    F-BAC gB/gD631 (49)201 (15)271 (21)194 (15)1,297
    F-BAC gD289 (19)72 (5)325 (21)836 (55)1,522
    F-BAC gB149 (15)2 (0.2)141 (14)714 (71)1,006
VD60/gB47 cells
    F-BAC (w.t.)162 (18)090 (10)647 (72)899
    F-BAC gB/gD225 (20)7 (0.6)145 (13)738 (66)1,115
  • a Vero or VD60/gB47 cells were infected with wild-type (w.t.) F-BAC, F-BAC gB/gD, F-BAC gD-kan (F-BAC gD), or F-BAC gB for 19 to 28 h and then fixed and processed for electron microscopy. Virus particles were counted in 10 to 20 representative cells.