TABLE 2

Yeast two-hybrid screening for direct interaction of KHC and KLC with AC141 or VP39

pBD constructpAD constructGrowth of colonies ond:
Leu- and Trp-deficient mediumLeu-, Trp-, and His-deficient medium
EmptyEmptya+
KHCKLCb++
KLCEmptyc++
KHCEmpty
KLCAC141++
TPRAC141+
ΔAC141KLC+
ΔAC141TPR+
KLCVP39++
TPRVP39+
VP39KLC+
VP39TPR+
KHCAC141+
Stalk/tailAC141+
ΔAC141KHC+
ΔAC141Stalk/tail+
KHCVP39+
Stalk/tailVP39+
VP39KHC+
VP39Stalk/tail+
  • a Negative control.

  • b Positive control.

  • c Autotransactivation.

  • d +, growth; −, no growth.