TABLE 1

Mutagenesis primers for generating recombinant MuNoV strains

TABLE 1
  • a Ten to 15 clones of the ORF2 gene encoding the MNV-1 VP1 protein were sequenced from the fecal material of individual persistently infected mice fed either a 2% or 20% protein diet (n = 10 mice total), as described by Hickman et al. (22). Seventeen nonsynonymous mutations were detected in total. Rows describing S domain residues are shaded yellow, rows describing P1 domain residues are shaded blue, and rows describing P2 domain residues are shaded red. We introduced each mutation into an MNV-1 infectious clone. The underlined mutations were viable and thus investigated further in this study. The asterisk (*) indicates a mutation that was introduced into the parental MNV-3 infectious clone.

  • b The frequency of clones in individual mice containing the specified mutation compared to inoculum; the number of mice containing this mutation.

  • c Sense (S) and antisense (AS) primers, all listed 5′ to 3′, were used to introduce the mutations indicated by bold and underlining into the parental MNV-1 infectious clone.