TABLE 6

Proteins in giant phages with similarity to SPN3US proteins that are encoded by genes identified as having an amber mutation in this study or expected to be essential

Phage% identity of match to SPN3US proteina:
gp75bgp256bgp260bgp19gp25gp29gp47gp64gp70gp78gp112gp168gp171gp186gp203gp214gp219gp238gp241gp244c
PhiEaH288858769785671294977448163807865757888
CR56367705142273826314643574744535971
ϕKZ2426342024162022302124222930
201ϕ2-12325342123102324291423213428
ϕPA32327331525152023301422213129
Ea35-702826342425231622352522223030
PhiEaH1222731172623152022292017243027
RSL22328311821161624241322253230
JM-2012222531172214?24231822202826
VP4B21212820221220222418172624
OBP23202922251319222418202618
EL21222526251318212418182516
ϕR1-3728252117
AR92813232115
BpSp2614242019
  • a Similarity was determined using PSI-BLAST with a maximum of three iterations.

  • b Three proteins were included as positive controls, as they must be essential in all myoviruses and are expected to be detectable in related phages. They are gp75 (major capsid), gp256 (sheath protein), and gp260 (terminase).

  • c The newly identified candidate vRNAP subunit, gp244, in SPN3US and its homologs are also included.