TABLE 2

Amber mutation sites in mixtures containing two SPN3US amber mutants whose particles were combined in amounts differing by 6.25-fold

MixtureMutantMutation positionaSNP %SPN3US ORFDNA changebAmino acid changec
1am2118685685.70214c.682C→Tp.Q228.
am2820397513.80238c.1186C→Tp.Q396.
2am2415909982.30171c.361C→Tp.Q121.
am3019069815.70219c.271C→Tp.Q91.
  • a Position relative to that of GenBank accession number JN641803.1. Amber mutation positions were confirmed in each mutant by Sanger sequencing.

  • b “c.” indicates the coordinate of the mutation within the open reading frame and its reference to called base change.

  • c Periods after position numbers indicate that the glutamine at that position in the polypeptide chain is not replaced, as its codon has been mutated to a nonsense codon and the protein product truncated.