TABLE 1

Cytokine levels in joint tissue homogenates after CHIKV infectiona

CytokineGenotypeDay 1Day 2
Mean pg/ml (SEM)PMean pg/ml (SEM)P
IL-1αWT7.6 (±1.0)0.29.8 (±0.6)0.03
Ifitm3−/−12 (±2.4)14 (±1.5)
IL-1βWT52 (±11)0.3144 (±18)0.2
Ifitm3−/−71 (±14)181 (±9.2)
IL-2WT11 (±1.9)0.0315 (±1.9)0.09
Ifitm3−/−16 (±1.8)21 (±3.1)
IL-3WT0.33 (±0.06)0.90.39 (±0.08)0.006
Ifitm3−/−0.33 (±0.06)0.76 (±0.06)
IL-4WT3.2 (±0.2)0.34.2 (±0.5)0.1
Ifitm3−/−3.2 (±0.5)6.1 (±1.0)
IL-5WT0.6 (±0.2)0.93.0 (±1.0)0.7
Ifitm3−/−0.8 (±0.4)3.4 (±1.0)
IL-6WT1.5 (±0.4)0.88.8 (±1.1)0.4
Ifitm3−/−1.9 (±1.0)11 (±1.9)
IL-9WT22 (±7.1)0.931 (±9.1)0.006
Ifitm3−/−28 (±16)107 (±24)
IL-10WT1.1 (±0.06)0.33.1 (±0.9)0.02
Ifitm3−/−1.3 (±0.2)5.3 (±0.8)
IL-12p40WT1.1 (±0.2)0.810 (±1.6)0.02
Ifitm3−/−1.4 (±0.3)15 (±0.8)
IL-12p70WT2.8 (±0.2)0.16.2 (±0.7)0.5
Ifitm3−/−3.8 (±0.5)7.0 (±0.6)
IL-13WTLOD (38.7)0.9LOD (38.7)0.9
Ifitm3−/−44 (±5.7)39 (±0.8)
IL-17WT0.3 (±0.09)0.90.3 (±0.08)0.8
Ifitm3−/−0.3 (±0.1)0.2 (±0.06)
EotaxinWT151 (±3.8)0.5176 (±11)0.9
Ifitm3−/−162 (±8.2)176 (±12)
G-CSFWT0.7 (±0.1)0.94.2 (±1.2)0.007
Ifitm3−/−0.9 (±0.3)8.5 (±0.8)
GM-CSFWT43 (±4.9)0.1459 (±6.4)0.04
Ifitm3−/−55 (±5.0)77 (±5.7)
IFN-γWTLOD (1.2)>0.91.8 (±0.3)0.7
Ifitm3−/−LOD (1.2)1.5 (±0.14)
KCWT16 (±2.6)0.681 (±15)0.8
Ifitm3−/−23 (±5.6)83 (±13)
MCP-1WT52 (±15)0.01706 (±119)0.4
Ifitm3−/−118.5 (±25.44)833 (±70)
MIP-1αWT38 (±1.4)0.3168 (±29)0.8
Ifitm3−/−50 (±7.8)151 (±13)
MIP-1βWT20 (±3.9)0.3150 (±33)0.2
Ifitm3−/−34 (±8.5)89 (±23)
RANTESWT13 (±2.4)0.8109 (±28)0.8
Ifitm3−/−12 (±3.1)88 (±24)
TNF-αWT17 (±3.0)0.00448 (±7.5)0.5
Ifitm3−/−41 (±6.4)56 (±7.4)
  • a Mice were infected with 103 FFU of CHIKV-LR in the footpad. Ipsilateral joint tissues were collected at 1 and 2 days after infection, homogenates were prepared, and the indicated cytokines were measured by Bio-Plex array. Data represent the means (±standard errors of the means) in picograms per milliliter from 9 to 11 mice per group. Statistical significance was determined by the Mann-Whitney test. LOD, limit of detection.