TABLE 1

Summary of GPCMV CRISPR-Cas9 mutagenesis

GPCMV geneHomolog in HCMVaNo. of plaquesNo. of WT plaquesMutant frequencyNo. of unique mutantsNo. of NSb mutantsFrequency of NSb mutantsNo. of TR recombinantsc
gp3US221010.90650.830
GP23UL231020.80860.750
GP24UL241130.73740.571
GP28UL281220.83760.860
GP28.1UL291110.91970.781
GP29UL291201.00870.880
GP29.1UL291220.83760.860
GP133UL1312001.0012100.830
gp139US221001.00970.780
gp141US23801.00771.000
gp142US241201.00950.561
gp143US221101.00760.860
gp144US2619100.47720.292
gp145TRS1/IRS11110.91920.220
gp146TRS1/IRS11210.92860.750
MCSN/A1650.69430.750
Total197280.86124890.725
  • a Based on homology to HCMV genes (49, 50). N/A, not applicable.

  • b NS, nonsense.

  • c Mutants with apparent recombination between the Cas9 cleavage site and the terminal repeat (TR) sequences.