TABLE 1

Results of epitope binding competition assaya

Detecting MAbb% competition of bindingc with the following competing antibody:
FVM01pFVM02pFVM04FVM09FVM13FVM20c6D8
FVM02p-biotinNone83NoneNoneNoneNoneNone
FVM04-biotinNoneNone8224NTNoneNone
FVM20-biotinNoneNone23969677None
KZ52-biotinNTNoneNone53NT17None
m13C6NTNone76NoneNTNoneNone
m2G4NoneNoneNoneNoneNoneNoneNone
m4G7NoneNoneNoneNoneNoneNoneNone
m16G8None23NoneNoneNoneNoneNone
m8C4None1819NoneNoneNoneNone
m17C6NoneNone91NoneNoneNoneNone
m4B8NoneNone20NoneNoneNoneNone
  • a The antigen (EBOV GPΔTM) coated on ELISA plate was first incubated with the competing antibody at 20 μg/ml before adding the detecting antibody at its respective EC50. The percent competition of binding was calculated by comparison to an irrelevant competing antibody. Antibody binding was detected using antistreptavidin antibody conjugated to HRP for the biotinylated antibodies and HRP-conjugated anti-mouse antibodies for untagged primary MAbs.

  • b The prefix “m” before the MAb indicates the murine origin of the antibody.

  • c None, less than 15% reduction in binding; NT, not tested.