TABLE 3

Peptides identified by LC-MS in the CK2-phosphorylated VP8

CK2 concnSiteIdentified peptidePeptide phosphorylation status
PhosphorylatedNonphosphorylated
Probability (%)aNo. of spectrabProbability (%)No. of spectra
3.3 ng/μlT107GPNGHAGDT107DAPPER1002010064
GPNGHAGDT107DAPPERAPEGGAPQDYLTAHLR81110031
S137AIEALPES137APHR100610037
S221LS221EGPPLLNMEAAAAAAGER981100136
DERLS221EGPPLLNMEAAAAAAGER10030
S240S240VVEELFTYAPAQPQVEVPLPR99210078
S679LRPVAS679PPLAGK100210014
≤3.3 pg/μlT107GPNGHAGDT107DAPPER100210035
GPNGHAGDT107DAPPERAPEGGAPQDYLTAHLR010018
S137AIEALPES137APHR95210022
S221LS221EGPPLLNMEAAAAAAGER0100154
DERLS221EGPPLLNMEAAAAAAGER01005
S240S240VVEELFTYAPAQPQVEVPLPR010070
S679LRPVAS679PPLAGK010029
  • a The highest probability score is used.

  • b Number of spectra in which the peptide was identified.