TABLE 2

Quantitation of DilC18(5)-DS (Dil) lipophilic dye-stained GFP-capsid-associated particles in the PNS of HSV-infected cellsa

Virus-cell (concn of Dil [μM])bMean no. (± SD) of particles per fieldDil-positive green particles as % of total
Total greenDil-positive green
K26-V (0)1,189 ± 6414 ± 31.2 ± 0.3
K26-V (10)1,194 ± 86333 ± 5227.9 ± 3.5
K26-H (0)1,220 ± 7716 ± 41.3 ± 0.4
K26-H (10)1,314 ± 120389 ± 4529.6 ± 3.1
GS-V (0)1,195 ± 2318 ± 21.5 ± 0.2
GS-V (10)1,254 ± 112354 ± 3628.2 ± 2.8
GS-H (0)1,221 ± 7317 ± 41.4 ± 0.3
GS-H (10)1,221 ± 79336 ± 4827.5 ± 3.4
  • a For each concentration of dye, counting was done in five independent microscopic fields from K26-V and K26-H and in seven fields from GS-V and GS-H to determine the mean particle number per field.

  • b V, Vero cells; H, HS30 cells; K26, HSV-1 strain K26GFP; GS, HSV1-GS2491.