TABLE 1

Quantitation of DiD lipophilic dye-stained GFP-capsid-associated particles in the PNS of HSV-infected cellsa

Virus-cell (concn of DiD [μM])bMean no. (± SD) of particles per fieldDiD-positive green particles as % of total
Total greenDiD-positive green
K26-V (0)1,108 ± 17813 ± 31.2 ± 0.3
K26-V (10)1,211 ± 286226 ± 6518.7 ± 4.7
K26-H (0)1,131 ± 9614 ± 51.2 ± 0.4
K26-H (10)1,195 ± 143257 ± 2721.5 ± 2.9
GS-V (0)1,062 ± 12515 ± 41.4 ± 0.4
GS-V (10)1,073 ± 240228 ± 4921.2 ± 5.6
GS-H (0)1,085 ± 10214 ± 41.3 ± 0.4
GS-H (10)1,086 ± 160216 ± 4619.9 ± 4.5
  • a For each concentration of dye, counting was done in 12 independent microscopic fields from K26-V and K26-H and in 11 fields from GS-V and GS-H to determine the mean particle number per field.

  • b V, Vero cells; H, HS30 cells; K26, HSV-1 strain K26GFP; GS, HSV1-GS2491.