TABLE 3

Apparent affinity and maximal binding of MAbs to EBOV WT GP and EBOV Δmucin GPa

MAbEBOV WT GPEBOV Δmucin GP
Kd,app (nM)Bmax signal (105)Kd,app (nM)Bmax signal (105)
KZ521.9 ± 0.54.7 ± 0.61.9 ± 0.826 ± 0.4
2G47.0 ± 3.813 ± 2.72.8 ± 0.439 ± 9.0
4G73.3 ± 0.56.6 ± 2.61.3 ± 0.326 ± 5.0
13C61.2 ± 0.217 ± 1.70.4 ± 0.139 ± 3.9
1H312.7 ± 4.06.6 ± 1.04.2 ± 0.326 ± 5.7
6D80.5 ± 0.132 ± 0.0NDND
13F60.9 ± 0.435 ± 3.7NDND
V5NA20 ± 0.4NA39 ± 1.49
  • a The values for apparent affinity (Kd,app) and maximal binding (Bmax, shown as relative fluorescence units) were derived from the ELISAs using VLPs pseudotyped with EBOV WT GP or EBOV Δmucin GP, as shown in Fig. 8. Values are averages (and ranges) of two experiments. NA, not applicable; ND, not detectable.