TABLE 1

Residues critical for EBOV MAb bindinga

TABLE 1
  • a Summary data for EBOV GP MAbs are shown. MAb reactivities for each alanine scan mutant are expressed as a percentage of wild-type reactivity with ranges (half of the maximum minus minimum values) in parentheses. Values for critical residues are shaded in gray. At least two replicate values were obtained for each experiment. The infectivity of each mutant and neutralization (Neut) titer of each MAb were determined using lentiviral reporter pseudotypes. The percent sequence identity at individual residues was determined by comparing sequences representing the five Ebolavirus species (EBOV, BDBV, TAFV, SUDV, and RESTV). The ability of each MAb to detect GP by Western blotting (an indication of the conformational or linear nature of its epitope) is indicated in references 27 and 28. NN, nonneutralizing.