TABLE 1

Summary of clade C Vpu mutants with respect to their ability to enhance virion release and downregulate BST-2

β-TrCP binding siteP value for impairment in virion release vs wild-type VpuaAbility to downregulate BST-2 vs ΔVpu
IntactMutated
L78A<0.05Yes
R79ANSYes
L80A<0.05Yes
L81ANSYes
LR/AANSYes
LL/AA<0.05Yes
LRLL/AAAA<0.05Yes
LRLL/HAPWNSYes
S58,62N<0.05No
L78A + S58,62NNSNo
R79A + S58,62N<0.05No
L80A + S58,62N<0.05No
L81A + S58,62N<0.01No
LR/AA + S58,62N<0.01No
LL/AA + S58,62N<0.01No
LRLL/AAAA + S58,62N<0.01No
LRLL/HAPW + S58,62N<0.05No
  • a NS, not significant.