TABLE 1

Characteristics of the VP6 peptides assigned to contact RNA

Samplem/zError (ppm)Assign.Confid.a (%)Peptide sequencePosition (aa)ModificationsFold increase above area with no HCHOb
reVP6/ssRNA655.394.797.8SAAILLAPGDVIK2–14Acetylation, N-terminal>10
reVP6/ssRNA733.444.6100SAAILLAPGDVIKR2–15Acetylation, N-terminal>10
reVP6/dsRNA557.804.4100IHTAVGSGSGTK83–94None>10
reVP6/ssRNA and reVP6/dsRNA851.905.3100VGGGGGDADAGVGATGTNGGR111–131None>10, 5.6
reVP6/ssRNA608.340.5100WVVLTEEIAR132–141None>10
Core/dsRNA682.030.4100IDVYRDEVPAQIIEVER151–167None>10
Core/dsRNA789.682.4100IDVYRDEVPAQIIEVERSLQKELGISR151–177None>10
Core/dsRNA699.370.4100DEVPAQIIEVERSLQKELGISR156–177None>10
reVP6/ssRNA and dsRNA795.37–3.6100EGVEEEKTSEEPAR221–234None>10
reVP6/ssRNA and dsRNA646.353.5100IGITIEGVMSQK235–246Oxidation of M9>10
reVP6/ssRNA and dsRNA545.801.6100GVMSQKKLLSMIGGVERKMA241–260Oxidation of M11>10
reVP6/ssRNA, dsRNA, and core609.851.7100KLLSMIGGVER247–257Oxidation of M5>10
reVP6/ssRNA and dsRNA545.802.2100LLSMIGGVER248–257Oxidation of M45.2, 5.2
reVP6/ssRNA and dsRNA647.341.3100ESAVMLVSNSIK266–277Oxidation of M5>10
reVP6/ssRNA and dsRNA588.321.5100ESAVMLVSNSIKDVVR266–281Oxidation of M5>10
reVP6/ssRNA and dsRNA554.94–0.1100ATAYFTAPTGDPHWK282–296None>10
Core/dsRNA706.69–1.683.9ATAYFTAPTGDPHWKEVAR282–300None>10
Core/dsRNA669.850.1100NILAYTSTGGDVK307–319None>10
Core/dsRNA784.89–0.8100NILAYTSTGGDVKTE307–321None>10
Core/dsRNA853.121.1100NILAYTSTGGDVKTEFLHLIDHL307–329None>10
reVP6/ssRNA473.944.5100IGITIEGVMSQKK235–247None>10
reVP6/ssRNA609.85–2.682.9LLSMIGGVERK248–258None>10
reVP6/ssRNA558.822.993.5VSNSIKDVVR272–281None>10
  • a Confidence of peptide assignment from collision-induced peptide fragmentation.

  • b The area of the peptide peak relative to the area for the reaction performed without formaldehyde.