TABLE 3

Mutations in BG505.T332N Env pseudovirusesa

Position(s)Mutation(s)Glycan(s)Relative dilution factorb
Parental (BG505.T332N)1.0
130Q130NN130-KI0.50
130, 241S241N + Q130NN130-KI, N241-KI0.25
197N197AN197-KO0.050
234N234SN234-KO0.20
234, 241S241N + N234SN234-KO, N241-KI1.8
241S241NN241-KI0.10
241, 278S241N + T278AN241-KI, N276-KO0.50
241, 291S241N + P291TN241-KI, N289-KI0.30
241, 332S241N + N332TN241-KI, N332-KO0.30
241, 355, 356S241N + NN355DKN241-KI, N355-KO0.10
278T278AN276-KO0.60
332N332TN332-KO3.4
355NN355DKN355-KO0.50
358, 360I358Tc+ R360IN356-KIc0.45
465T465NN465-KI1.4
  • a Other than the T332N change, the mutations used to create the parental BG505 SOSIP.v4.1 trimers (SOS, I559P, and the v4.1 mutations E64K and A316W [13, 15, 27]) were not made in the corresponding BG505 pseudoviruses.

  • b The pseudoviruses were diluted to give infectivities corresponding to luminescence values of ∼2 × 106 RLU in the Tzm-bl assay. The dilution factors yielding such signals are relativized to that of the parental virus.

  • c This mutation adds a sequon adjacent to the N355 one; glycan occupancy on the two Asn residues is uncertain.