TABLE 2

Kinetic constants for nucleotide incorporation by FMDV 3D-wt and 3D(M16A)a

sym/subNTP3D-wt3D(M16A)
Kd,app (μM)kpol (s−1)kpol/Kd,app (μM−1 s−1)Kd,app (μM)kpol (s−1)kpol/Kd,app (μM−1 s−1)
sym/sub-AUA452505.615553.7
sym/sub-UGC1530020.01023023.0
sym/sub-UAU562203.9542003.7
sym/sub-ACG1225012.81515010.0
  • a Kinetic parameters obtained from data shown in Fig. 3, according to the procedures detailed in Materials and Methods. Kd,app, dissociation constant for NTP-binding; kpol, optimal polymerization rate constant; kpol/Kd,app, catalytic efficiency.