TABLE 1

Mutant spectrum analysis of FMVD-CS8c1 and FMDV-3D(M16A) passaged in the absence and presence of ribavirin (+R)a

FMDV region, passage no.bNo. of nucleotides analyzed (no. of molecular clones/no. of haplotypes)cNo. of different mutations (total no. of mutations)dMutation frequency (Mf)e
Minimum (Mfmin)Maximum (Mfmax)
CS8-c1, p124,080 (28/6)5 (5)2.1 × 10−42.1 × 10−4
CS8-c1 + R, p125,800 (30/17)28 (28)1.1 × 10−31.1 × 10−3
3D(M16A), p126,660 (31/8)7 (7)2.6 × 10−42.6 × 10−4
3D(M16A) + R, p126,660 (31/25)32 (32)1.2 × 10−31.2 × 10−3
CS8-c1, p445,762 (78/9)7 (7)1.5 × 10−41.5 × 10−4
CS8-c1 + R, p443,552 (73/34)49 (58)1.1 × 10−31.3 × 10−3
3D(M16A), p443,552 (73/16)17 (17)3.9 × 10−43.9 × 10−4
3D(M16A) + R, p442,692 (72/33)37 (67)8.6 × 10−41.6 × 10−3
  • a The regions analyzed comprise residues 7160 to 8019 of the 3D-coding region for passage 1 and residues 6610 to 7051 and 7160 to 8019 of the 3D-coding region for passage 4.

  • b The origins of FMDV CS8-c1 and FMDV-3D(M16A) and the passage (p) conditions in the absence or presence of ribavirin (+R) are described in the legend to Fig. 1 and Materials and Methods.

  • c The number of haplotypes represents the number of different RNA sequences.

  • d The number of different mutations and the total number of mutations were counted relative to the consensus sequence of the corresponding population.

  • e Mfmin and Mfmax were calculated with the number of different and total mutations, respectively. Both FMDV C-S8c1 and FMDV-3D(M16A) exhibited significant increases of Mfmin as a result of replication in the presence of ribavirin: P < 0.0005 and P = 0.0001 for the differences at passage 1 between FMDV C-S8c1 and FMDV-3D(M16A) in the absence and presence of ribavirin, respectively; P < 0.0001 and P = 0.0079 for the corresponding differences at passage 4; χ2 test. Other differences for Mfmin were not statistically significant (P = 0.0502 to P = 0.9103; χ2 test).