TABLE 2

Clinical responses of swine exposed to combination adenovirus treatment and FMDV challenge depend on time of challenge

GroupTreatmentAd titer (TCID50)Time of challengea (dpi)Animal no.Maximum amt of virus detected in (DPC of first detection)b:Maximum clinical score (DPC of first detection)
SerumMouth swab
1-1Ad-null control6 × 1092201.2 × 107 ± 0.2 × 107 (2)1.75 × 107 ± 0.1 × 108 (1)11(3)
212.8 × 108 ± 0.8 × 108 (1)6.8 × 107 ± 0.6 × 107 (1)12(4)
226.4 × 107 ± 0.3 × 107 (1)2.9 × 107 ± 0.2 × 107 (1)12(4)
1-2Ad-null control1.5 × 10107231.9 × 106 ± 0.5 × 106 (4)1.2 × 107 ± 0.5 × 107 (1)11(4)
1-3PBS7242.3 × 107 ± 0.1 × 107 (5)2.2 × 106 ± 0.9 × 106 (2)12(5)
251.2 × 108 ± 0.2 × 108 (1)1.3 × 106 ± 0.4 × 106 (1)11(2)
2Ad-porcine IFN-αγ1.2 × 109226NegcNegNeg
and Ad-3siRNAand 6 × 10927Neg6.4 × 105 ± 0.3 × 105 (7)Neg
281.3 × 107 ± 0.4 × 107 (4)6.2 × 106 ± 0.2 × 106 (5)6 (7)
3Ad-porcine IFN-αγ1.2 × 1094291.2 × 107 ± 0.1 × 107 (5)2.7 × 106 ± 0.1 × 106 (6)3 (7)
and Ad-3siRNAand 6 × 109301.1 × 106 ± 0.1 × 106 (3)5.0 × 106 ± 0.1 × 106 (2)10 (5)
311.8 × 102 ± 0.1 × 102 (7)NegNeg
4Ad-porcine IFN-αγ1.2 × 1097321.3 × 106 ± 0.2 × 106 (3)1.5 × 106 ± 0.3 × 106 (3)9 (6)
and Ad-3siRNAand 6 × 109331.2 × 107 ± 0.7 × 107 (3)2.6 × 107 ± 0.1 × 107 (3)8 (5)
346.1 × 105 ± 0.8 × 105 (4)7.5 × 105 ± 0.6 × 105 (4)6 (6)
5Ad-porcine IFN-αγ2.5 × 109435NegNegNeg
and Ad-3siRNAand 1.5 × 101036NegNegNeg
37NegNegNeg
6Ad-porcine IFN-αγ2.5 × 1097388.4 × 102 ± 0.1 × 102 (7)3.6 × 106 ± 0.6 × 106 (6)Neg
and Ad-3siRNAand 1.5 × 1010392.8 × 105 ± 0.6 × 105 (7)8.4 × 104 ± 0.7 × 104 (6)5(7)
402.3 × 106 ± 0.5 × 106 (5)9.0 × 106 ± 0.2 × 106 (5)9(6)
  • a Pigs were challenged with FMDV at 2, 4, or 7 dpi with adenovirus (or PBS).

  • b The data (FMDV RNA copy number per milliliter) represent means ± standard deviations from triplicate real-time RT-PCR experiments.

  • c Neg, no viral RNA and no clinical signs were detected.