TABLE 1

Clinical responses in swine exposed to combination adenovirus treatment and FMDV challenge depend on adenovirus titers

GroupTreatmentaAd titer (TCID50)Animal no.Maximum amt of virus detected in (DPC of first detection)b:Maximum clinical score (DPC of first detection)
SerumMouth swab
1Ad-null control6 × 109106.2 × 106 ± 0.2 × 106 (3)6.6 × 106 ± 0.3 × 106 (3)12 (4)
111.4 × 103 ± 0.1 × 103 (2)8.3 × 105 ± 0.4 × 105 (2)7 (2)
2Ad-porcine IFN-αγ2 × 109124.5 × 102 ± 0.7 × 102 (5)2.0 × 105 ± 0.1 × 105 (4)6 (3)
and Ad-3siRNAand 2 × 109131.1 × 105 ± 0.2 × 105 (9)2.7 × 104 ± 0.2 × 104 (8)1 (9)
14NegcNegNeg
3Ad-porcine IFN-αγ6 × 10915NegNegNeg
and Ad-3siRNAand 6 × 10916NegNegNeg
17dNDeNDND
  • a Recombinant adenoviruses were inoculated 24 h before FMDV challenge.

  • b The data (FMDV RNA copy number per milliliter) represent means ± standard deviations from triplicate real-time RT-PCR experiments.

  • c Neg, no viral RNA and no clinical symptoms were detected.

  • d Pig no. 17 died from a digestive disease during the experiment.

  • e ND, not detected.