TABLE 1

Primers used to introduce mutations into the UL38 coding sequence

Mutant typePrimer sequencea
SL/AA5′-CCACTCGGCCTCGTCCAGCGCGGCCATGATAGCGGCAGTGCTATG-3′
5′-GCCGCGCTGGACGAGGCCGAGTGG-3′
LD/AA5′-TCTGTCGCCACTCGGCCTCGGCCGCCAGGCTCATGATAGCGGCA-3′
5′-GCGGCCGAGGCCGAGTGGCGACAGA-3′
E/A5′-GCGTCTGTCGCCACTCGGCCGCGTCCAGCAGGCTCATGATAGC-3′
5′-GCGGCCGAGTGGCGACAGACGC-3′
EW/AA5′-CGTCCATCTGCGTCTGTCGCGCCGCGGCCTCGTCCAGCAGGC-3′
5′-GCGGCGCGACAGACGCAGATGGACG-3′
RQ/AA5′-CCCCCACGTCCATCTGCGTCGCTGCCCACTCGGCCTCGTCCA-3′
5′-GCAGCGACGCAGATGGACGTGGGGG-3′
TQ/AA5′-TCAGTCCCCCCACGTCCATCGCCGCCTGTCGCCACTCGGCCT-3′
5′-GCGGCGATGGACGTGGGGGGACTGA-3′
  • a Underlined letters indicate nucleotide substitutions introduced into the UL38 coding sequence.