TABLE 1.

MAb reactivity, endo H sensitivity, and cell surface expression of HN glycosylation mutant viruses

VirusReactivity to MAba:endo H sensitivitybCell surface expressionc
15C410D11B79AVS
rBC+++++SYes
rG1++++++++SYes
rG2++SYes
rG3++++++++SYes
rG4+++SYes
rG12+++++++SYes
  • a The reactivities of the HN-specific MAbs to the HN protein of NDV were tested by an ELISA. The data are presented as the relative amounts of HN captured by each of the MAbs. ++++, A450, >2.0; +++, A450, >1.1; ++, A450, 0.9 to 1.0; +, A450, 0.5 to 0.8; −, A450, <0.4. Data are the results from three independent experiments.

  • b S, carbohydrate side chains were sensitive to endo H digestion. Data are the results from three independent experiments.

  • c Cell surface expression was analyzed by indirect immunofluorescence with an anti-HN MAb cocktail. Data are the results from three independent experiments.