TABLE 3.

Binding activities of Fab monoclonal antibodies to each of the four DENV serotypes as determined by ELISAa

FabELISA titer of Fab binding to:
DENV-1DENV-2DENV-3DENV-4
1A54.13.83.83.8
3A24.13.83.83.8
2H74.13.83.63.8
1B23.93.83.83.8
1A104.13.83.63.8
5H2<1.0<1.0<1.03.8
3E44.03.83.63.8
1F2<1.0<1.0<1.0<1.0
  • a Microtiter plates were coated with DENV-1, DENV-2, DENV-3, or DENV-4 virions. Data are presented as log10 of the reciprocal dilution that gave an optical density reading twofold or higher than the background. DENV cross-reactive Fab 3E4- and DENV-4-specific Fab 5H2 were described previously (23). The starting concentration of each Fab was approximately 140 μg/ml. Chimpanzee Fab 1F2 was used as a negative control.