TABLE 1.

Listing of potential expressed ORFs in SGIV

ORFNucleotide position (length [aaa])MWb (kDa)pIcConserved domain or signatured (CD/Prosite accession no.)MatchPredicted structure and/or functiong
BlastP score% IdentityAccession no.eSpeciesf
ORF001L1971-1057 (304)34.659.19TM
ORF002R1502-1903 (133)14.208.86
ORF003R2018-3163 (381)43.168.993-Beta hydroxysteroid dehydrogenase (pfam01073)38653AAL77802TFV054L3-beta-hydroxy-delta 5-C27-steroid oxidoreductase-like protein, TM
ORF004L4332-3235 (365)41.678.264523AAP33232ATV053R
ORF005L5542-4400 (380)40.408.72Transmembrane amino acid transporter protein (pfam01490) C-type lectin domain signature (PS00615)76.323AAF61849Mus musculusN system amino acids transporter NAT-1, TM, SA
ORF006R5570-6349 (259)29.155.2532467AAP33234ATV055REarly 31-kDa protein, TM
31869CAA07475EHNV
31868AAL77797TFV025R
26167CAA37177FV3
11532NP_078713LCDV122R
39.322AAL98842ISKNV118L
ORF007L7339-6416 (307)30.126.96TM
ORF008L7886-7194 (230)22.159.52
ORF009L8444-7980 (154)16.436.14TM
ORF010L8888-8517 (123)13.615.13
ORF011L9132-8944 (62)6.638.44TM, SP
ORF012L12293-9219 (1024)117.369.466435AAP33240ATV061R
ORF013R11173-11595 (140)14.496.08TM
ORF014L12773-12348 (141)15.857.987935AAP33239ATV060R
ORF015L13000-12821 (59)6.579.113351AAP33181ATV004RTM, SA
ORF016L14289-13048 (413)46.265.0832540AAP33180ATV003RTM
70.526AAK82090CIV229L
ORF017R13172-13609 (145)16.077.04TM
ORF018R14317-15174 (285)32.326.0217838AAP33179ATV002L
61.234NP_078687LCDV093R
ORF019R15196-16224 (342)36.827.44Poxvirus proteins of unknown function (pfam03003)38170AAP33178ATV001LTM
Glycoprotein hormones beta chain signature (PS00261)16437NP_078745LCDV160L
14533AAK82199CIV337L
ORF020L17246-16278 (322)35.378.6663.2040AAP33250ATV070L
ORF021L17725-17306 (139)16.426.1916159AAP33251ATV071L
14959AAK54494RRV
62.835NP_078640LCDV036R
ORF022L18277-17777 (166)18.5712.55
ORF023R17793-18290 (165)18.225.43TM
ORF024L18774-18319 (151)17.155.754527AAP33254ATV073L
ORF025L20488-18956 (510)56.497.31SAP, putative DNA-binding (bihelical) motif (smart00513)16153AAP33256ATV075L
15348AAK54496RRV
10036NP_078703LCDV110L
ORF026R20567-22267 (566)63.326.4037537AAP33257ATV076RTM
78.221NP_078649LCDV048R
ORF027L21162-20671 (163)17.088.94TM
ORF028L23363-22350 (337)36.677.99Ig-like domain (PS50385)TM, SP
ORF029L24445-23447 (332)36.586.42Neural cell adhesion molecule L1 (KOG3513)TM, SP
Ig-like domain (PS50385)
ORF030L25635-24610 (341)37.909.073337NP_041033EHV 1Tegument protein, TM, SP
ORF031L27160-26144 (338)37.628.46Ig-like domain (PS50385)TM, SP
ORF032L27516-27391 (41)4.649.82TM, SP
ORF033L29760-28726 (344)37.568.75Ig-like domain (PS50385)TM, SP
ORF034L30161-29823 (112)12.668.90TM, SP
ORF035L31388-30261 (375)42.268.99Neural cell adhesion molecule L1 (KOG3513)48.125BAC11344Homo sapiensUnnamed protein product, TM, SA
Ig-like domain (PS50385)
ORF036L32515-31526 (329)37.294.95TM, SP
ORF037L33696-32668 (342)37.128.77Neural cell adhesion molecule L1 (KOG3513)43.127CAA40912Drosophila melanogasterFibroblast growth factor receptor, TM, SP
ORF038L34236-33724 (170)19.049.7212164AAP33260ATV079LTM, SA
69.353NP_078769LCDV194R
36.241AAB94443CIV117L
ORF039L37417-34262 (1051)118.228.38Protein kinase domain (PS50011)42536AAP33261ATV080LTM
17645NP_078619LCDV010L
15028NP_078677LCDV080R
96.324AAK82240CIV380R
65.123AAL98779ISKNV055L
ORF040R36123-36698 (191)20.519.56TM, SP
ORF041L37978-37547 (143)15.246.05
ORF042R38058-38285 (75)8.476.23TM, SP
ORF043R38285-40288 (667)73.665.3617938AAP33262ATV081R
ORF044L39213-38608 (201)21.719.65TM
ORF045L41090-40362 (242)22.949.80
ORF046L41866-41120 (248)23.779.88
ORF047L43063-41909 (384)43.585.69Ribonucleotide reductase, beta subunit (COG0208)58071AAP33216ATV038RRibonucleoside-diphosphate reductase beta subunit, TM
56770AAL77807TFV071L
33148NP_078636LCDV027R
ORF048L43489-43214 (91)10.508.73Caspase recruitment domain (pfam00619)5742AAP33218ATV040LCARD-like caspase
ORF049L44002-43535 (155)17.066.58dUTPase (KOG3370)12346AAL77806TFV068RdUTPase
12047AAP33220ATV042L
12140AAK82298CIV438L
ORF050L44695-44033 (220)23.558.68Tumor necrosis factor receptor domain (cd00185)8232P25119Mus musculusTumor necrosis factor receptor superfamily member 1B precursor, TM, SP
ORF051L45563-44868 (231)26.127.43Tumor necrosis factor receptor domain (cd00185)7230AAO89081Mus musculusTumor necrosis factor receptor superfamily member 14 precursor, TM, SP
ORF052L48673-45767 (968)109.887.71Predicted ATPase (COG3378)141469AAP33258ATV077LD5 family NTPase, TM
Poxvirus D5 protein (pfam03288)60735NP_078717LCDV128L
19239AAB94479CIV184R
36546AAL98833ISKNV109L
ORF053R46254-46832 (192)20.359.99Lipoprotein lipid attachment site (PS00013)TM
ORF054R48777-49424 (215)25.134.9826568AAP33259ATV078RTM
10134AAL98780ISKNV056L
81.341NP_078618LCDV006L
80.527AAB94419CIV067R
ORF055R49447-50169 (240)22.7710.65Collagens (type XV) (KOG3546)80.933ZP_00122019Haemophilus somnus 129PTHypothetical protein
ORF056R50198-50938 (246)22.936.48
ORF057L54510-51004 (1168)131.308.83117652AAP33249ATV069RTM
116552AAK37740RRV
34026NP_078748LCDV163R
13423AAL98800ISKNV076L
80.131AAK82156CIV295L
ORF058R52463-52876 (137)13.759.22
ORF059L55000-54560 (146)16.329.104525AAP33185ATV008RTM, SP
ORF060R54967-57879 (970)109.658.98DNA repair protein, SNF2 family (KOG0390)121461AAL77795TFV009LPutative NTPase 1, TM
Helicase conserved C-terminal domain (pfam00271)121261AAP33184ATV007L
94058AAK53744RRV
74942NP_078720LCDV132L
41432AAL98787ISKNV063L
81.330AAD48148CIV022L
ORF061R57914-58528 (204)23.269.54Catalytic domain of CTD phosphatases (smart00577)18750AAP33244ATV064R
TFIIF-interacting CTD phosphatase (KOG1605)91.734AAL98729ISKNV005L
76.633NP_078678LCDV082L
53.527AAK82216CIV355R
ORF062R58593-59363 (256)27.695.18Insulin-like growth factor (smart00078)91.338BAC67672Cyanidioschyzon merolaeDNA-directed RNA polymerase II largest subunit, SP
Predicted DNA-binding protein (KOG2588)
ORF063L59278-58649 (209)22.163.88Transcription elongation factor (COG5164)56.247XP_220553Rattus norvegicusSimilar to charcot-marie-tooth duplicated region transcript I, TM
ORF064R59415-61133 (572)63.728.15Ribonucleotide reductase, alpha subunit (COG0209)82268AAL77800TFV041RRibonucleoside-diphosphate reductase, alpha subunit-like protein, TM
82768AAP33245ATV065R
63654NP_078756LCDV176L
ORF065R61268-61510 (80)9.358.85
ORF066R61603-61845 (80)8.709.30TM, SA
ORF067L62482-61907 (191)21.586.49Mitochondrial thymidine kinase 2 (KOG4235)15843AAP33196ATV019LDeoxynucleoside kinase, TM
Deoxynucleoside kinase (pfam01712)96.730NP_078725LCDV136R
56.226AAF44495FPV
53.125CAC84464SFAV-1
48.924CAC84481HVAV-3c
ORF068L63334-62516 (272)29.618.59Immunoglobulins and major histocompatibility complex proteins signature (PS00290)20950AAP33197ATV020LTM
10027AAL98836ISKNV112R
10027NP_078615LCDV003L
40.432AAK82296CIV436L
ORF069L64967-63321 (548)61.889.6221136AAP33194ATV017R
69.721NP_078643LCDV039R
ORF070R64994-65452 (152)17.039.32Erv1/Alt family (pfam04777)14347AAP33193ATV016LThiol oxidoreductase, TM
69.332NP_078699LCDV106L
65.537AAL98767ISKNV043L
38.926AAK82208CIV347L
ORF071R65483-66307 (274)31.618.585036AAP33192ATV015L
ORF072R66404-67795 (463)50.536.33Iridovirus major capsid protein (pfam04451)89599AAM00286GIVMajor capsid protein, TM
68171AAK55105TFV096R
67670AAO32315EHNV
67570AAP33191ATV014L
67071AAB01722FRG3V
48150AAC24486.2LCDV147L
41745AAK82135CIV274L
37443AAL72276ISKNV006L
ORF073L71185-67874 (1103)123.398.28RNA polymerase II, second largest subunit (KOG0214)153065AAL77805TFV065LDNA-directed RNA polymerase II second-largest subunit, TM
153365AAP33221ATV043R
129566AAK84400RRV
101846NP_078633LCDV025L
75541AAL98758ISKNV034R
ORF074R68472-68738 (88)9.168.527173AAP33222ATV043bL
5147NP_149893CIV430R
ORF075R71239-71775 (178)19.964.5044.337NP_078763LCDV185R
ORF076L72715-71858 (285)30.357.17Purine nucleoside phosphorylase (KOG3984)30550NP_000261Homo sapiensPurine nucleoside phosphorylase, TM
ORF077L73747-72839 (302)34.129.05TM
ORF078L76227-73855 (790)88.168.75Putative lipopolysaccharide-modifying enzyme (smart00672)55243AAL77799TFV029RPutative tyrosine protein kinase, TM
55343AAP33237ATV058R
23133NP_078770LCDV195R
17740AAB94478CIV179R
ORF079R74231-74680 (149)15.137.78TM, SP
ORF080R75872-76324 (150)15.939.10TM
ORF081L76809-76246 (187)21.799.0519849AAL77799TFV029RPutative tyrosine protein kinase
19147AAP33237ATV058R
14350AAK54490RRV
86.332NP_078770LCDV195R
70.928AAB94478CIV179R
ORF082L77592-76924 (222)24.329.02
ORF083R77672-79009 (445)50.489.2630842AAP33203ATV026LTM
ORF084L80193-79066 (375)41.649.17dsRNA-specific ribonuclease (COG0571)29445AAL77809TFV085LRibonuclease III, TM
Ribonuclease III family (smart00535)28745AAP33202ATV025R
21545NP_078726LCDV137R
12035AAB94459CIV142R
10837AAL98811ISKNV087R
ORF085R80251-80529 (92)10.578.27Transcription elongation factor TFIIS (COG1594)98.255AAL77810TFV086RTranscription elongation factor SII
95.955AAP33201ATV024L
5760BAA04187CV
5760AAC96492PBCV-1
56.642NP_078754LCDV171R
ORF086R80591-81055 (154)17.118.0810840AAA43825FV3Putative immediate-early protein, TM
10035AAB47251IMRV
10035AAP33200ATV023L
99.838AAL77811TFV087R
99.437AAB47252OMRV
ORF087R81385-82032 (215)25.226.64SP
ORF088L84187-82667 (506)54.014.8954655AAK54492RRVTM
54255AAP33230ATV051L
17631AAL98731ISKNV007L
17630BAC66967RSBI
17229NP_078665LCDV067L
10829AAB94444CIV118L
82.426CAC19148Ascovirus DpA V4
59.321AAK82318CIV458R
ORF089L85420-84248 (390)45.598.1749.323AAP33232ATV053R
ORF090L86627-85506 (373)43.487.745622AAP33232ATV053R
ORF091L87886-86750 (378)44.546.93
ORF092L89216-88086 (376)44.127.1833.519AAP33232ATV053R
ORF093L90497-89280 (405)47.597.3043.921AAP33232ATV053R
ORF094L91083-90622 (153)16.247.92TM
ORF095R90635-91111 (158)16.615.12TM, SP
ORF096R91148-91618 (156)16.887.53Tumor necrosis factor receptor domain (cd00185)45.435AAB53707Ranus norvegicusTumor necrosis factor receptor superfamily, member 11b Osteoprotegerin, TM, SP
ORF097L92774-91626 (382)43.189.03Xeroderma pigmentosum G, N, and I regions (cd00128)40051AAL77816TFV101RPutative DNA repair protein RAD2, TM
39452AAP33187ATV010L
38254AAK53745RRV
16133NP_078767LCDV191R
13028AAL98751ISKNV027L
12828BAA82754RSBI
94.723AAK82229CIV369L
ORF098R92428-93231 (267)30.519.4919156AAP33188ATV011RTM, SP
18756AAK53746RRV
10651CAC19143Ascovirus DpAV4
10240NP_078627LCDV019R
10150AAL98810ISKNV086L
90.542AAK82168CIV307L
ORF099R93244-93492 (82)9.075.13
ORF100L94153-93740 (137)14.899.13TM
ORF101R93753-94694 (313)35.036.13TM
ORF102L95007-94774 (77)8.546.93Ubiquitin/60s ribosomal protein L40 fusion (KOG0003)13481AAD44040BVDV-2Polyprotein
Ubiquitin/40s ribosomal protein S27a fusion (KOG0004)
ORF103R95092-95385 (97)11.055.044246AAP33269ATV088LTM, SA
ORF104L99252-95446 (1268)139.168.30RNA polymerase II, large subunit (KOG0260)147760AAL77794TFV008RDNA-dependent RNA polymerase largest subunit-like protein, TM
147659AAP33183ATV006R
94742AAA92868LCDV016L
71937BAA82753RSBI
70937AAL98752ISKNV028L
33231AAB33907CIV176R
ORF105R95498-95731 (77)8.1110.024045AAK82205CIV344RTM
ORF106R97298-98146 (282)29.9410.94TM
ORF107R99308-100453 (381)39.063.90TM, SP
ORF108L100309-99329 (326)33.4911.74TM
ORF109L100305-99400 (301)27.583.54TM, SP
ORF110L101067-100504 (187)21.216.29TM
ORF111R100766-101533 (255)29.235.4714833AAP33270ATV089R
5622NP_078768LCDV193L
ORF112R101588-102655 (355)34.666.09Collagens (type XV) (KOG3546)52.464BAB34267Escherichia coli O157:H7Putative tail fiber protein, TM, SP
ORF113L102633-101944 (229)21.616.54TM
ORF114L103050-102712 (112)11.988.92TM, SP
ORF115R103122-103580 (152)17.246.78B-Cell lymphoma (smart00337)4032AAF89533Ovis ariesBak protein, TM
ORF116R103700-104476 (258)30.139.1127758AAP33272ATV091RPutative replication factor
14440NP_078747LCDV162L
99.433AAK82143CIV282R
45.826AAL98785ISKNV061L
ORF117L104733-104575 (52)6.288.46
ORF118R104795-105754 (319)35.678.7727555AAP33268ATV087R
8527NP_078701LCDV108L
40.423AAK82148CIV287R
39.329AAL98820ISKNV096L
ORF119R105799-106050 (83)9.099.363629AAP33205ATV028L
ORF120L106525-106103 (140)15.968.8911047AAP33204ATV027R
10847AAK84402RRV
55.532NP_078638LCDV032R
ORF121R106615-106869 (84)9.848.23TM, SP
ORF122L107599-106967 (210)24.259.48
ORF123L108740-107652 (362)41.488.6516929AAL13097RGV9807TM
16928AAP33224ATV045R
ORF124R108863-109399 (178)20.196.54TM, SP
ORF125R109474-110028 (184)21.085.92TM, SP
ORF126R110101-110658 (185)20.628.00TM, SP
ORF127R110731-111252 (173)19.887.14TM, SP
ORF128R112041-115070 (1009)114.958.63DNA polymerase family B (pfam00136)137465AAL77804TFV063RDNA polymerase, TM
137365AAP33223ATV044L
64437NP_078724LCDV135R
54063AAK54493RRV
53134AAL98743ISKNV019R
53134BAA28669RSBI
45434CAC84133Iridovirus RMIV (IV22)
36230CAC19127Ascovirus DpAV4
31531AAD48150CIV037L
26325CAC84471HVAV-3c
26126CAC19170SFAV-1
25868AAK84401RRV
ORF129L115490-115308 (60)6.684.57
ORF130L116083-115673 (136)14.896.91TonB-dependent receptor proteins signature (PS00430)
ORF131R115749-116303 (184)19.934.53IG-like domain (PS50385)TM, SP
ORF132R116321-117148 (275)31.369.5019848AAP33263ATV082RTM
ORF133R117168-117440 (90)9.563.57
ORF134L118498-117527 (323)36.508.33ATPases (smart00382)42874AAL77796TFV016RATPase
36966AAA43823FV3
43074AAP33264ATV083L
28858NP_078656LCDV054R
25953AAL98847ISKNV122R
25953AAL68652GIV
25953BAA28670RSBI
25953BAA96406SBIV
25953BAA96407GIV
25953AAL68653GSIV
25953AAL68654LBIV
25953AAO16492LYCIV
25953BAA96408ALIV
21755AAL73346SCV
21655AAN77575SOV
20156AAM00905RSBI
19442AAB94422CIV075L
ORF135L118885-118547 (112)12.955.988543AAP33265ATV084L
3322NP_078646LCDV042L
ORF136R118946-119260 (104)11.617.66Possible membrane-associated motif in LPS-induced tumor necrosis factor alpha factor (smart00714)97.861AAP33206ATV029RTM
ORF137R119282-120667 (461)49.6910.0711435AAP33207ATV030R
ORF138L120907-120713 (64)7.384.72
ORF139R121013-121324 (103)11.3410.02
ORF140R121397-122311 (304)32.144.85
ORF141R122567-124558 (663)69.454.59Extracellular matrix glycoprotein Laminin subunit beta (KOG0994)80.125AAK01205Mus musculusMage-d3, TM
ORF142L124134-122572 (520)54.733.46TM, SP
ORF143L124882-124643 (79)8.878.553449AAP33212ATV035LTM, SA
ORF144R124963-125421 (152)16.679.07Fibroblast growth factor (KOG3885)67.833NP_570107Rattus norvegicusFibroblast growth factor, SP
ORF145R125480-125977 (165)18.079.20Acidic and basic fibroblast growth factor family (cd00058)47.429NP_032028Mus musculusFibroblast growth factor, TM, SP
ORF146L127052-126078 (324)36.716.72Predicted E3 ubiquitin ligase (KOG1814)26942AAP33208ATV031RNTPase/helicase
26543AAL77808TFV078L
10530NP_078700LCDV107R
60.835AAC97709MSEPV
ORF147L128221-127187 (344)39.405.494227AAP33224ATV045RTM
4025AAL13097RGV 9807
ORF148R128324-128803 (159)17.636.9911745AAP33210ATV033L
54.328NP_078659LCDV059L
ORF149R128843-129220 (125)14.595.10
ORF150L130827-129301 (508)57.287.8828334AAP33226ATV047LPhosphotransferase, TM
17127NP_078729LCDV143L
65.935AAL98737ISKNV013R
39.727AAK82240CIV380R
ORF151L131435-130848 (195)22.266.487535AAP33227ATV048LTM
4529NP_078686LCDV091R
ORF152R131534-132772 (412)46.579.46DNA or RNA helicases of superfamily II (COG1061)33444AAP33229ATV050RHelicase, SP
DEAD-like helicases superfamily (smart00487)32844CAB37349EHNV
32444AAK55107TFV056L
16030AAB94470CIV161L
64.724NP_042815ASFV
ORF153L132661-132089 (190)21.968.935830CAB37350EHNV40kDa protein
5729CAA58035FV3
ORF154R132788-133081 (97)10.879.73TM
ORF155R133172-134899 (575)64.635.02Semaphorins (KOG3611)13526NP_035482Mus musculusSema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A; H-Sema K1; Semaphorin K1, TM, SP
ORF156L135860-135048 (270)31.125.61
ORF157R135948-136472 (174)19.738.8018055AAP33225ATV046L
ORF158L136944-136528 (138)15.776.15
ORF159R137020-137511 (163)17.429.69TM, SP
ORF160L137996-137508 (162)18.905.6012240AAP33238ATV059R
77.437NP_078685LCDV090R
ORF161R138345-138533 (62)6.966.28TM, SP
ORF162L139822-138674 (382)44.126.5240750AAP33190ATV013LImmediate-early protein ICP-46
40350AAK53747RRV
40149AAL77815TFV097R
36247PI4358FV3
13127NP_078648LCDV047L
48.529AAK82253CIV393L
45.420AAL98839ISKNV115R
  • a aa, amino acid.

  • b MW, molecular mass.

  • c pI, isoelectric point.

  • d Accession numbers starting with PS are Prosite-derived numbers, while those starting with cd, smart, pfam, COG, or KOG are CD-Search within BlastP-derived numbers.

  • e Accession numbers are from GenBank or SwissProt database.

  • f All species corresponding virus ORFs are listed; only best match is listed if species are not related to virus.

  • g Function was deduced from the degree of amino acid similarity to or products of known genes or by the presence of Prosite signatures; TM, transmembrane domains; SP, N-terminal signal peptide; SA, N-terminal signal anchor.