TABLE 2.

Real-time PCR data obtained per individual ChIP experiment

Virus and DNA targetExpt no.Bound CtcBound quantityaInput CtInput quantityaBound/input ratiobBound/input ratio relative to LAT promoter bound/input ratio
KOS/M
    LAT promoter36.571.0E-0434.135.4E-04
136.778.7E-0534.703.7E-04
36.758.8E-0534.464.3E-04
    Avg ± SD9.2E-05 ± 7.1E-064.5E-04 ± 8.9E-050.21 ± 0.041 ± 0.30
35.332.4E-0434.135.4E-04
235.402.3E-0434.703.7E-04
35.462.2E-0434.464.3E-04
    Avg ± SD2.3E-04 ± 1.0E-054.5E-04 ± 8.9E-050.51 ± 0.101 ± 0.30
34.474.3E-0432.721.4E-03
334.294.9E-0432.591.6E-03
35.052.9E-0432.351.9E-03
    Avg ± SD4.0E-04 ± 1.0E-041.6E-03 ± 2.2E-040.25 ± 0.071 ± 0.41
32.142.2E-0331.613.1E-03
431.982.4E-0332.162.1E-03
31.722.9E-0331.673.0E-03
    Avg ± SD2.5E-03 ± 3.7E-042.7E-03 ± 5.4E-040.91 ± 0.221 ± 0.35
    LAT 5′ exon35.426.2E-0434.869.1E-04
136.183.7E-0435.008.3E-04
NDND35.227.1E-04
    Avg ± SD5.0E-04 ± 1.8E-048.2E-04 ± 9.9E-050.61 ± 0.232.96 ± 1.29
34.391.3E-0334.869.1E-04
233.512.3E-0335.008.3E-04
NDND35.227.1E-04
    Avg ± SD1.8E-03 ± 7.3E-048.2E-04 ± 9.9E-052.16 ± 0.934.73 ± 2.03
33.642.1E-0334.661.0E-03
333.612.1E-0334.351.3E-03
34.591.1E-0334.491.2E-03
    Avg ± SD1.8E-03 ± 5.9E-041.2E-03 ± 1.2E-041.52 ± 0.536.17 ± 2.78
32.026.3E-0331.449.3E-03
432.066.1E-0331.797.3E-03
32.265.3E-0331.837.1E-03
    Avg ± SD5.9E-03 ± 5.0E-047.9E-03 ± 1.2E-030.74 ± 0.130.82 ± 0.25
    ICP0 promoter41.692.4E-0634.384.6E-04
141.243.3E-0634.424.5E-04
41.123.6E-0634.444.4E-04
    Avg ± SD3.1E-06 ± 6.4E-074.5E-04 ± 1.0E-050.01 ± 0.000.04 ± 0.01
37.196.1E-0534.384.6E-04
238.023.4E-0534.424.5E-04
37.196.1E-0534.444.4E-04
    Avg ± SD5.2E-05 ± 1.6E-054.5E-04 ± 1.0E-050.12 ± 0.040.23 ± 0.08
39.321.3E-0533.131.1E-03
339.998.2E-0633.807.0E-04
38.941.7E-0534.036.0E-04
    Avg ± SD1.3E-05 ± 4.6E-068.1E-04 ± 2.9E-040.12 ± 0.010.07 ± 0.04
434.623.9E-0431.922.7E-03
35.102.8E-0431.733.1E-03
35.162.6E-0432.252.1E-03
    Avg ± SD3.1E-04 ± 6.9E-052.7E-03 ± 4.9E-040.12 ± 0.030.13 ± 0.05
KOS/29
    LAT promoter34.34.8E-0428.892.1E-02
133.936.2E-0428.812.2E-02
34.195.2E-0428.762.2E-02
    Avg ± SD5.4E-04 ± 7.3E-052.2E-02 ± 9.7E-040.03 ± 0.001 ± 0.20
33.67.8E-0428.892.1E-02
233.478.6E-0428.812.2E-02
33.269.9E-0428.762.2E-02
    Avg ± SD8.8E-04 ± 1.0E-042.2E-02 ± 9.7E-040.04 ± 0.011 ± 0.18
32.351.9E-0328.892.1E-02
333.329.5E-0428.812.2E-02
32.311.9E-0328.762.2E-02
    Avg ± SD1.6E-03 ± 5.4E-042.2E-02 ± 9.7E-040.07 ± 0.031 ± 0.49
32.242.0E-0330.317.7E-03
432.51.7E-0330.407.2E-03
31.553.2E-0330.466.9E-03
    Avg ± SD2.3E-03 ± 8.3E-047.3E-03 ± 3.8E-040.32 ± 0.111 ± 0.51
    LAT 5′ exon32.943.4E-0331.886.9E-03
132.654.1E-0331.767.5E-03
33.312.6E-0331.399.6E-03
    Avg ± SD3.4E-03 ± 7.4E-048.0E-03 ± 1.4E-030.42 ± 0.1216.6 ± 5.29
34.221.4E-0331.886.9E-03
233.22.8E-0331.767.5E-03
33.462.4E-0331.399.6E-03
    Avg ± SD2.2E-03 ± 7.2E-048.0E-03 ± 1.4E-030.27 ± 0.106.72 ± 2.65
31.867.0E-0331.886.9E-03
333.732.0E-0331.767.5E-03
33.482.3E-0331.399.6E-03
    Avg ± SD3.8E-03 ± 2.8E-038.0E-03 ± 1.4E-030.47 ± 0.366.42 ± 5.41
32.325.1E-0331.021.2E-02
431.966.5E-0331.379.8E-03
32.285.3E-0331.151.1E-02
    Avg ± SD5.6E-03 ± 7.8E-041.1E-02 ± 1.3E-030.51 ± 0.091.59 ± 0.64
    ICP0 promoter37.365.4E-0530.011.1E-02
137.475.0E-0530.081.0E-02
37.594.6E-0530.209.4E-03
    Avg ± SD5.0E-05 ± 4.1E-061.0E-02 ± 6.9E-040.00 ± 0.000.20 ± 0.04
35.891.6E-0430.011.1E-02
235.941.5E-0430.081.0E-02
35.741.7E-0430.209.4E-03
    Avg ± SD1.6E-04 ± 1.2E-051.0E-02 ± 6.9E-040.02 ± 0.000.39 ± 0.06
37.445.1E-0530.011.1E-02
338.921.8E-0530.081.0E-02
38.821.9E-0530.209.4E-03
    Avg ± SD2.9E-05 ± 1.9E-051.0E-02 ± 6.9E-040.00 ± 0.000.04 ± 0.03
33.677.7E-0430.447.9E-03
434.414.5E-0430.806.1E-03
34.155.5E-0430.656.8E-03
    Avg ± SD5.9E-04 ± 1.6E-046.9E-03 ± 9.1E-040.10 ± 0.030.27 ± 0.13
  • a Quantity determined by using the Ct (y) to determine quantity (x) based on a standard curve generated for the primer and probe set used.

  • b Bound/input ratio, average bound quantity/average input quantity.

  • c ND, not determined.