TABLE 1.

Real-time PCR primers and probes

DNA targetSequenceAccession no. (nucleotide no.)
UL54 (ICP27)5′ GCC CGT CTC GTC CAG AAG 3′ (forward)X14112.1 (113945-114034)
5′ GCG CTG GTT GAG GAT CGT T 3′ (reverse)
5′ CAG CAC CCA GAC GCC 3′ (probe)
LAT promoter (LAP1)5′ CAA TAA CAA CCC CAA CGG AAA GC 3′ (forward)X14112.1 (118263-118350)
5′ TCC ACT TCC CGT CCT TCC AT 3′ (reverse)
5′ TCC CCT CGG TTG TTC C 3′ (probe)
5′ LAT exon5′ GGC TCC ATC GCC TTT CCT 3′ (forward)X14112.1 (119326-119397)
5′ AAG GGA GGG AGG AGG GTA CTG 3′ (reverse)
5′ TCT CGC TTC TCC CC 3′ (probe)
ICP0 promoter5′ CCG CCG ACG CAA CAG 3′ (forward)X14112.1 (124494-124578)
5′ GTT CCG GGT ATG GTA ATG AGT TTC T 3′ (reverse)
5′ CTT CCC GCC TTC CC 3′ (probe)
HSV DNA Pol5′ AGA GGG ACA TCC AGG ACT TTG T 3′ (forward)X14112.1 (65880-65953)
5′ CAG GCG CTT GTT GGT GTA C 3′ (reverse)
5′ ACC GCC GAA CTG AGC A 3′ (probe)
Mouse Xist5′ GCT CTT AAA CTG AGT GGG TGT TCA 3′ (forward)M97167.1 (862-930)
5′ GTA TCA CGC AGA AGC CAT AAT GG 3′ (reverse)
5′ ACG CGG GCT CTC CA 3′ (probe)
Mouse centromere5′ TGG CGA GGA AAA CTG AAA AAG GT 3′ (forward)X06899.1 (66-136)
5′ TTT TCT TGC CAT ATT CCA CGT CCT A 3′ (reverse)
5′ CAG TGG ACA TTT CTA AAT TT 3′ (probe)