TABLE 4.

Expression of WSSV structural genes during the WSSV infection cycle

Gene no.WSSV ORFNameExpression (hpi)Calibrated expression ratio at time (hpi) showna
TFPeak0246812182436
1419VP66418240.01440.03030.01530.03430.01850.03460.05790.10440.0569
252VP18024240.02170.02430.00960.03570.04460.05960.0490.14560.0971
3326VP136A12360.00970.01720.00650.01830.01260.02840.14530.67450.8101
4524VP136B12240.00590.01580.0020.00830.01340.01780.04050.10120.0535
592VP11012240.00890.01450.00630.01610.01430.02790.01580.04530.0297
6502VP9518240.00910.00590.00810.00660.00830.01280.02660.06060.0485
7388VP7512240.00220.001100.00320.0050.01010.09360.10560.0655
8275VP732360.00160.004700.00190.0020.00310.00250.00440.0075
9381VP60A6240.00380.00790.00540.01450.00580.02120.03210.07120.0589
10474VP60B8240.00750.01890.00380.02960.02190.03050.02730.12350.0489
1151VP552360.00470.01570.00770.00890.01020.02070.02820.05320.0711
1267VP53A2240.00760.05870.02170.0340.03920.02880.01590.07130.0578
13171VP53B8360.00720.02040.00680.01570.02380.01470.01170.03780.0419
14324VP53C2360.00050.00180.00340.0010.00440.00330.01380.01950.034
15294VP51A8360.00110.00220.001700.00340.00470.0090.01340.0242
16311VP51B18240.00160.00090.00030.00460.00220.00270.03720.0580.0547
17364VP51C23600.00520.00120.00070.00370.00340.02140.03340.0346
18293VP41A18240.0020.00140.00150.000200.00450.01460.05010.0498
19298VP41B24360.00550.0080.0020.00530.00640.00830.00870.02420.0668
20362VP39A24360.00240.000100.00060.00320.00130.00180.01050.0238
21395VP39B24360.00270.001500.00340.00410.00460.00720.01680.0299
22314VP38A36360.01720.0010.0020.00250.00360.00240.01260.02620.0688
23449VP38B12240.02560.0440.01640.07070.04210.0940.0950.26790.1216
24134VP36A18360.00150.00130.00030.00180.00270.00130.01860.05560.0946
25309VP36B18360000000.02130.0280.0387
2619VP3512240.01820.01640.00090.02450.03970.05480.38460.62980.385
27253VP3218360.00270.00230.0010.00190.00540.00360.00850.02950.0381
28396VP3124360.00270.002100.000200.00080.0070.01530.0317
29480VP2812360.00340.00170.00310.00510.00490.01940.14710.36990.7033
30367VP2612360.00710.01740.00580.01090.00980.03080.19350.24210.8689
3158VP2418360.00790.01270.00580.00850.01370.01570.03380.0620.2099
32359VP2212240.00110.00170.00210.00230.00210.00430.00350.01760.0168
33473VP1912360.0110.02010.01190.02380.02880.05160.15780.39330.6816
34269VP1512360.00110.00060.00210.0020.00280.01820.13480.25210.4087
35339VP13A2360.00050.00680.00580.00180.00540.00330.00760.01410.0148
36377VP13B12240.00480.00650.00430.00650.00910.02280.04740.09010.0681
37445VP12B2360.01470.06150.02580.02270.03930.05160.09820.19540.4168
3865VP12A8240.0002000.00040.00150.00450.0180.04660.0394
39394VP1118360.00320.00530.00350.00160.0040.0050.02470.03950.0806
  • a The calibrated ratio is the ratio of the expression of the WSSV structural gene in infected shrimp gill cells compared to a universal control probe (actin). TF is the time (hpi) at which the baseline (0 hpi) expression level increased by at least threefold. Peak expression always occurred at either 24 or 36 hpi.