TABLE 2.

5′ and 3′ UTR sequence analysis and microarray transcriptional expression levels of the WSSV structural genes

VP no.NameWSSV ORFTATA boxaInr motifbKozak contextcPoly(A) signaldTranscriptional expression levele
1VP664WSSV419ΔS+L
2VP180WSSV052Δ+S+L
3VP136AWSSV326Δ+SH
4VP136BWSSV524++W+M
5VP110WSSV092+SL
6VP95WSSV502Δ+W+L
7VP75WSSV388ΔS+M
8VP73WSSV275ΔSL
9VP60AWSSV381+S+M
10VP60BWSSV474WM
11VP55WSSV051Δ+S+L
12VP53AWSSV067+W+L
13VP53BWSSV171ΔS+L
14VP53CWSSV324Δ+SH
15VP51AWSSV294Δ+W+M
16VP51BWSSV311++S+M
17VP51CWSSV364++W+H
18VP41AWSSV293Δ+WM
19VP41BWSSV298+SL
20VP39AWSSV362Δ+WL
21VP39BWSSV395+WL
22VP38AWSSV314Δ+W+L
23VP38BWSSV449Δ+WL
24VP36AWSSV134++S+H
25VP36BWSSV309Δ+S+H
26VP35WSSV019++S+M
27VP32WSSV253Δ+WL
28VP31WSSV396Δ+WL
29VP28WSSV480Δ+S+H
30VP26WSSV367+S+H
31VP24WSSV058Δ+W+M
32VP22WSSV359++SM
33VP19WSSV473++S+H
34VP15WSSV269++W+H
35VP13AWSSV339+SM
36VP13BWSSV377Δ+SM
37VP12BWSSV445+W+M
38VP12AWSSV065+S+H
39VP11WSSV394Δ+S+M
  • a The presence of a TATA box (TATAAA) is indicated by +. The presence of a truncated sequence (TATA or TATAA) is indicated by Δ.

  • b The presence of an Inr motif (A/TCAC/G/TT) upstream of the translation start codon (ATG) is shown by +.

  • c The Kozak context (PuNNATGPu) of the initiation codon is either S (strong) or W (weak).

  • d The presence of a 3′ UTR poly(A) signal (AATAAA) is indicated by +.

  • e Microarray transcriptional expression levels are shown as follows: low (L), baseline mRNA levels increased by a factor of between 3 and 15; medium (M), mRNA increased by a factor of 15 to 60; high (H), increased by a factor of 60 to >300.