TABLE 1.

Effect of stem-loop B on thermostability of encapsidated hph 215-507 RNAa

RNATm (°C)% initial dimers
PRPR+PRPR+
215-50743 ± 1.552 ± 1.562 ± 7.579 ± 18
215-507Δ16<3751.5 ± 2.235 ± 868 ± 7
  • a The Tms of hph 215-507 dimers with or without the deletion of nucleotides 290 to 305 were determined following extraction from either ψ or PR ψ virions. The table shows means and standard deviations for Tm and for percent dimers in the sample incubated at 37°C. A Tm could not be obtained for the PR sample containing the deletion because it contained <50% dimers following incubation at 37°C.