TABLE 2.

Antiviral activity of plant lectins against HIV-1 strains selected for resistance to GNA and HHA

Test compoundEC50 (μg/ml) for virusa:
HIV-1(IIIB)GNA-1.0 (0.5)GNA-1.1a (10)GNA-1.2 (100)GNA-2.1 (100)HHA-1.1 (1)HHA-1.3 (10)HHA-2.1 (20)HHA-2.2 (100)
GNA0.40 ± 0.141.0 ± 0.012.2 ± 5.248.8 ± 8.528.8 ± 2.52.8 ± 1.820 ± 0.05.5 ± 2.131.0 ± 12.3
HHA0.28 ± 0.171.2 ± 0.510.0 ± 3.237.5 ± 9.621.0 ± 10.52.0 ± 0.711 ± 1.46.1 ± 5.518.8 ± 10.4
NPA0.40 ± 0.140.90 ± 0.1430.0 ± 14.143.3 ± 5.836.7 ± 5.86.0 ± 2.819 ± 1610.0 ± 2.826.7 ± 5.8
CA0.65 ± 0.216.0 ± 0.035.0 ± 7.170.0 ± 42.455.0 ± 7.18.0 ± 2.820 ± 0.017.5 ± 10.637.5 ± 3.5
LOA0.08 ± 0.00.2 ± 0.01.7 ± 0.76.5 ± 0.73.4 ± 0.90.80 ± 0.283.3 ± 1.10.75 ± 0.351.3 ± 0.35
Cyanovirin0.019 ± 0.0120.056 ± 0.0340.12 ± 0.06
2G12MAb1.6 ± 0.57>50>50
DS-50000.60 ± 0.200.90 ± 0.851.1 ± 0.450.90 ± 0.420.95 ± 0.100.70 ± 0.420.55 ± 0.350.45 ± 0.070.85 ± 0.51
T-200.087 ± 0.0600.035 ± 0.0210.12 ± 0.100.038 ± 0.0040.13 ± 0.110.06 ± 0.010.55 ± 0.350.075 ± 0.0350.029 ± 0.019
AMD31000.050 ± 0.0140.075 ± 0.0350.49 ± 0.570.20 ± 0.070.15 ± 0.110.13 ± 0.100.05 ± 0.00.11 ± 0.070.12 ± 0.07
UC-7810.0016 ± 0.00050.0021 ± 0.00120.0080 ± 0.00240.0043 ± 0.00250.0030 ± 0.00.002 ± 0.00.003 ± 0.00.0035 ± 0.00070.0028 ± 0.0011
Tenofovir3.3 ± 1.11.6 ± 0.67.9 ± 2.96.3 ± 3.54.7 ± 1.22.3 ± 0.84.9 ± 1.22.3 ± 0.04.0 ± 3.2
  • a EC50, compound concentration required to inhibit virus-induced syncytium formation in CEM cell cultures by 50%. With the exception of HIV-1(IIIB), HIV-1 strains were isolated in the presence of different concentrations of GNA and HHA. The code numbers of the various virus isolates correspond to the gp120 sequencing data shown in Table 1 (first column).