TABLE 2.

Rescue in cis of both EC tropism and leukocyte transfer of Huv Leuk viral variants showing reversal of UL131-128 mutations after adaptation to growth in HUVECs

HCMV strain (mutated gene)Rescue in cis of HUVEC tropism and leukocyte transfer after attempts at adaptation to growth in HUVECs (Huv+ Leuk+ rev) in:
HUVECaPMN/monocytesb
RVFIX mutants
    RVFIXΔUL132-12800/0
    RVFIXΔUL131K00/0
    RVFIXΔUL13000/0
    RVFIXΔUL128K00/0
Clinical isolates
    VR1814 Huv Leuk (UL131)808,800/8,200
    VR3480 Huv Leuk (UL130)807,000/7,300
    Merlin Huv Leuk (UL128)807,500/8,000
Laboratory strains
    AD169 Huv Leuk (UL131)8570/7,500
    Towne Huv Leuk (UL130)858,500/9,200
    Toledo Huv Leuk (UL131-128)00/0
  • a That is, the percentage of infected cells in the HUVEC monolayer after more than 10 passages.

  • b That is, the median number of pp65-positive leukocytes/2 × 105 leukocytes examined in five independent experiments. Individual figures were within a 30% range.