TABLE 1.

Epitopes and mutants

EpitopeSequenceMHCLikely effect of mutation(s)
Gag CM9CTPYDINQMA*01
Gag CM9 mutantaCAPYDINQMP2: secondary anchor
Tat SL8STPESANLA*01
Tat SL8 mutantPTPESANQP1: binding? P8: C-terminal anchor
Nef IW9IRYPKTFGWB*17
Nef IW9 mutantTRYPKIFGWP1: secondary anchor? P6: TCR
  • a Substitutions within Gag CM9 were associated with isoleucine-to-valine substitutions upstream and downstream of the epitope sequence. Since these substitutions were likely required to restore fitness to the Gag CM9 escape virus (10), they were included in the mutagenesis strategy. We identified no extraepitopic mutations occurring in association with the consensus variants of Tat SL8 or Nef IW9.