TABLE 1.

Biological activities of mutant HAs

HANo. of glycosylation sitesNo. of oligo- saccharidesCell surface expressionaBinding (% of WT)bCell fusionc
Guinea pig erythrocytesChicken erythrocytes
WT22+100100+
G111+111115+
G222+11099+
G333+9736+
G444+258+
G5a54+174+
G5b55+166+
G665+113+
  • a +, efficiency of transport to the cell surface identical to that of wild-type HA.

  • b Released hemoglobin is shown as a percentage of that of wild-type (WT) HA.

  • c +, activity comparable to that of wild-type HA.