TABLE 2.

Probes used for genotyping

OligonucleotideProbe typeaSequence (5′-)Peak melting temp (°C) for:Difference (°C)
Webster (E)Oka (J)
R1P1M1SCGC GCC ATG TAT TTA ACG GG63.8468.54.66
R1P2M1AAG AAC TGG CAC GGT TGC GAG ATA TGG
aR1P1M2AGCC GTT ACG GCA TTA GAT ACT GTG60.1169.08.89
sR1P2M2STC GCC ACA ATC CAC ATA CC
R1P1M3SAAT ATT CCA CCA CCT TTG G58.8361.802.97
R1P2M3ATG TTA AGA GGG TTA ACA YGG TTT G
R1P1M4AGTA TTC ACC GTT ATG TTT CCA GGT55.8063.307.50
R1P2M4STA GTA TTG AAG GAC TCC TTC
R1P1M-MexSCTA CAG CCG TTA CGG CA69.9869.983.00b
R1P2M-MexATA GAT ACT GTG TTT CGC CAC AAT CCA
  • a S, sensor probe; A, anchor probe.

  • b A difference in annealing temperatures is seen only in selected Mexican M strain variants (annealing temperature for the Mexican sample was 66.98°C).