TABLE 3.

Expression of IFN-α subtypes in L929 cells infected by NDV or RVFV or stimulated by poly(I · C)a

StimulusExposure time (h)No. of clones obtained by IFN subtype
IFN-α2IFN-α4IFN-α5IFN-α6TIFN-αψ3
NDV9224
NDV121191
RVFV9191
Poly(I · C)9125111
Poly(I · C)125185
  • a The proportions of the different IFN-α subtypes was analyzed in L929 cells. Total IFN-α was amplified by reverse transcription-PCR by using degenerated primers, as described previously (44). PCR products were then cloned and sequenced. Subtypes that are not shown in the table were not detected.