TABLE 2.

Summary of mutagenesis results

RepliconTranslation (%)Replication (%)aReversionb
1st bp
    79A-U1 (WT)100100
    79AxC19815Partial (C or U)
    79AxA110616Yes
    79AxG19614Yes
    79UxU1113<1ND
    79U-A1109<1ND
    79G-U199132No
2nd bp
    78G-C2 (WT)100100
    78G-U2102<1ND
    78GxA2101<1ND
    78GxG2115<1ND
    78CxC2113<1ND
3rd bp
    77A-U3 (WT)100100
    77AxC3108<1ND
    77AxA3107<1ND
    77AxG3105<1ND
    77UxU397<1ND
    77G-U39623No
    77U-A39856No
4th bp
    76U-A4 (WT)100100
    76UxU4120<1ND
    76U-C4107<1ND
    76U-G41046No
5th bp
    75C-G5 (WT)100100
    75CxA592<1ND
    75CxC5112<1ND
    75CxU5110<1ND
    75GxG584<1ND
    75AxG583<1ND
    75U-G5903.5No
    75G-C597<1ND
6th bp
    74U-G6 (WT)100100
    74UxC610127No
    74UxU611173No
    74U-A6112181No
  • a The translation and replication levels of mutant replicons are expressed as the replicons' Rluc activities as a percentage of the wild-type (WT) level at 2.5 and 72 h posttransfection, respectively.

  • b For reversion analysis, mutant replicons were extracted at 72 h posttransfection, polyadenylated, and subjected to 3′ RACE analysis (see details in Materials and Methods). ND, not determined.