TABLE 1.

Primers used in this study

Primer name5′ to 3′ sequencea
3′-BglII-ZeocinCCA AGA TCT TTA AAA ATG AAG TTT TAG CAC GTG TCA G
5′-BamHI-FRT-ZeocinACT GGA TCC GAA GTT CCT ATT CTC TAG AAA GTA TAG GAA CTT CCG TTT ACA ATT TCG CCT GAT GCG G
5′M50EKpnIGTA GGT ACC GAA GAC GTG TCG CGT CAT GAC CT
3′M50EKpnIGTA GGT ACC GGA ACG AGT CGA TCG TCG TCA G
5′M50KpnICGC GGT ACC ATG GAG ATC GAC A
3′M50XhoICGC CTC GAG TCA CGG ATG ACC
Del5′-forCGA AAG AAC TAT TTC CTT TTT GAC C
Del4-revGCC GGA AAT AGT TCC GCA GTC GGT GGG AAC G
Del3-revGCC GGA AAT AGT TCC CCA GCG CTG ACC CGG GGG AGG
Del2-revGCC GGA AAT AGT TCC CCC ACC TCC TGC GTA TCT TCG
Del1-revGCC GGA AAT AGT TCC GGC GGG GGG AGG CCG CTG GG
5′M50YAGTC GCT CCG GTC GAG GCT CTC CTC AGC TAT TGG
3′M50YAGAG AGC CTC GAC CGG AGC GAC GAT ATC GGT GGT
5′M50Y1AGTC GCT CCG GTC GAG TAT CTC CTC AGC TAT TGG
3′M50Y1AGAG ATA CTC GAC CGG AGC GAC GAT ATC GGT GGT
5′M50Y2AGTC TAT CCG GTC GAG GCT CTC CTC AGC TAT TGG
3′M50Y2AGAG AGC CTC GAC CGG ATA GAC GAT ATC GGT GGT
5′M50EAGTC TAT CCG GTC GCT TAT CTC CTC AGC TAT TGG
3′M50EAGAG ATA AGC GAC CGG ATA GAC GAT ATC GGT GGT
5′-ΔM50 GGC GAG GAT CAG GGC GCA GTG AAT AGC GTT GGC GTG CCT GGT CAA AAA GGA AAT AGT TCT CGA TTT ATT CAA CAA AGC CAC G
3′-ΔM50 GTC GGC TCG GGC GGC GCC ACT CGG ACG GCG GCG AGC TCA TCC GCG GCG GCG CCG GCG CGA GCC AGT GTT ACA ACC AAT TAA CC
  • a The sequences homologous to M50 for recombination are underlined.