TABLE 2.

Reconstitution of ΔM50 constructs

BACGeneaPlaque formationb
pSM3frM50+++
pΔm152/GFPM50+++
pSMfr-ΔM50ΔM50
pΔM50-GFPΔM50
pΔM50-GFP + pCR3-M50FlagΔM50 + M50 in trans(+)
pM50EΔM50 + M50 ectopic+++
  • a M50 expression and M50/p35 in trans.

  • b Formation of viral plaques 10 days postinfection. +++, wt plaque formation; −, no plaque formation; (+), formation of miniplaques.