TABLE 1.

Effect of WNV dose on mortality and survival time of B6 mice

Dosea (PFU/mouse)Total no. of micebNo. of dead miceSurvival time (days)Mortality (%)MSTc (days)
108301551005.6 ± 0.7
116
47
1073151005.7 ± 0.8
26
711012.0 ± 1.0
412
213
106101103011.0 ± 1.0
111
112
10510193010.0 ± 1.0
110
111
103305102710.5 ± 0.8
211
112
10210194012.0 ± 3.2
110
113
116
10100>210
1100>210
  • a Virus dose in 100 μl of HBSS injected via tail vein.

  • b All surviving mice seroconverted; WNV-specific antibodies were detected by enzyme-linked immunosorbent assay at day 21 p.i.

  • c Values represent MST ± standard deviation of mice which died up to 21 days p.i. Differences in mortality were analyzed byχ 2 test, and differences in MST were assessed by Student's t test (see Materials and Methods for details).