TABLE 2.

Summary of the properties of glycosylation mutants

VirusHCVcc infectivityaHCVpp infectivitya,bCore releasecSensitivity to neutralizationd
Wild type+++++++++
Mutant
    E1N1+/−++ND (+)
    E1N2++++ND (+)
    E1N3+++++++ND (+)
    E1N4++++/−ND (+)
    E2N1+++++++++
    E2N2++− (−)++++e
    E2N3+++++ND (+)
    E2N4++− (−)+++e
    E2N5+++++++
    E2N6+++++++++
    E2N7+/−+++ (+)+ND (−)
    E2N8+/−ND
    E2N9+++++++++
    E2N10ND
    E2N11+++/−++
  • a Percentage of infectivity: +++, >90%; ++, between 30% and 90%; +, between 10% and 30%; +/−, between 2% and 10%; −, <2%.

  • b Infectivity of HCVpp of genotype 1a as previously reported (19). Values in parentheses are results obtained for HCVpp of genotype 2a.

  • c Percentage of core release: ++, >75%; +, between 30% and 75%; +/−, between 12% and 30%; −, <12%.

  • d Sensitivity to antibody neutralization: +, similar to that of the wild type; ++, more than 5-fold increase in sensitivity to neutralization with most antibodies tested; −, decrease in sensitivity to neutralization. Results in parentheses were obtained with HCVpp of genotype 1a only (22). ND, not determined.

  • e Results obtained with the HCVcc system only.