TABLE 2.

Primers used for UL130ccta mutagenesis

Mutant BACPrimer directionPrimer sequencea
TB40-BAC4-UL130ccta48-51ForwardAGAATCCGTCCCCGCTATGGTCTAAACTGACGTATTCCGCACCGGCAGCAGCGGCGACGTTTTACTGTaggatgacgacgataagt
ReverseGGCGAGGGATAGATAAAAGGACAGTAAAACGTCGCCGCTGCTGCCGGTGCGGAATACGTCAGTTTAGAcaaccaattaaccaattctga
TB40-BAC4-UL130ccta82-86ForwardCAATTCTCGGGGTTCCAGCGGGTATTAACGGGTCCCGCATGTGCAAACGCAACCCTGTATCTGCTGTaggatgacgacgataagt
ReverseGGTCTGGCCTTCCCGGTTGTACAGCAGATACAGGGTTGCGTTTGCACATGCGGGACCCGTTAATACCcaaccaattaaccaattctga
TB40-BAC4-UL130ccta94-95ForwardTCCCGAGTGTCGCAACGAGACCCTGTATCTGCTGTACAACGCAGCAGGCCAGACCTTGGTGGAGAGaggatgacgacgataagt
ReverseTTTTCACCCATGTAGAGCTTCTCTCCACCAAGGTCTGGCCTGCTGCGTTGTACAGCAGATACAGGGcaaccaattaaccaattctga
TB40-BAC4-UL130ccta101-102ForwardCCTGTATCTGCTGTACAACCGGGAAGGCCAGACCTTGGTGGCAGCAAGCTCCACCTGGGTGAAAAAaggatgacgacgataagt
ReverseCGCTCAGGTACCAGATCACCTTTTTCACCCAGGTGGAGCTTGCTGCCACCAAGGTCTGGCCTTCCCcaaccaattaaccaattctga
TB40-BAC4-UL130ccta108-109ForwardGGAAGGCCAGACCTTGGTAGAGAGAAGCTCCACCTGGGTGGCAGCAGTGATCTGGTACCTGAGCGGaggatgacgacgataagt
ReverseGGAGGATGGTCTGGTTGCGACCGCTCAGGTACCAGATCACTGCTGCCACCCAGGTGGAGCTTCTCTcaaccaattaaccaattctga
TB40-BAC4-UL130ccta127-131ForwardAGCGGTCGCAACCAGACCATCCTCCAACGGATGCCCGCAACAGCTTCAGCACCGAGCGACGGAAACGaggatgacgacgataagt
ReverseGTCTTCCACGCTGATCTGCACGTTTCCGTCGCTCGGTGCTGAAGCTGTTGCGGGCATCCGTTGGAGGcaaccaattaaccaattctga
TB40-BAC4-UL130ccta142-145ForwardCTTCAAAACCGAGCGACGGAAACGTGCAGATCAGCGTGGCAGCAGCCGCAATTTTTGGAGCGCACATGaggatgacgacgataagt
ReverseAGCTTGGTCTGCTTGGGCACCATGTGCGCTCCAAAAATTGCGGCTGCTGCCACGCTGATCTGCACGTTcaaccaattaaccaattctga
TB40-BAC4-UL130ccta154-157ForwardTGGAAGACGCCAAGATTTTTGGAGCGCACATGGTGCCCGCACAGACCGCACTGCTACGCTTTGTAGTCaggatgacgacgataagt
ReverseTGATAACGTGTGCCATCGTTGACTACAAAGCGTAGCAGTGCGGTCTGTGCGGGCACCATGTGCGCTCCcaaccaattaaccaattctga
TB40-BAC4-UL130ccta165-168ForwardTGCCCAAACAGACCAAGCTGCTACGCTTCGTCGTCAACGCAGGCACAGCATATCAGATGTGTGTGATGaggatgacgacgataagt
ReverseTGAGCCCAGCTCTCCAGCTTCATCACACACATCTGATATGCTGTGCCTGCGTTGACGACGAAGCGTAGcaaccaattaaccaattctga
TB40-BAC4-UL130ccta181-185ForwardTATCAGATGTGTGTGATGAAGCTGGAGAGCTGGGCTGCAGTCTTCGCAGCATACAGCGTGTCTTTTCaggatgacgacgataagt
ReverseGGTGAACGTCAATCGCACCTGAAAAGACACGCTGTATGCTGCGAAGACTGCAGCCCAGCTCTCCAGCcaaccaattaaccaattctga
TB40-BAC4-UL130ΔPNLIVForwardCACCGAGGCCAATAACCAGACTTACACCTTCTGCACACATTGAGCCCGTCGCACGAGCAGaggatgacgacgataagtaggg
ReverseCGCGCGGTTTTCAAAATTCCCTGCTCGTGCGACGGGCTCAATGTGTGCAGAAGGTGTAAGcaaccaattaaccaattctgattag
TB40-BAC4-UL130addPNLIVForwardCACCGAGGCCAATAACCAGACTTACACCTTCTGCACACATCCCAATCTCATCGTTTGAGCCCGTCGCACGAGCAGaggatgacgacgataagtaggg
ReverseCGCGCGGTTTTCAAAATTCCCTGCTCGTGCGACGGGCTCAAACGATGAGATTGGGATGTGTGCAGAAGGTGTAAGcaaccaattaaccaattctgat
TB40-BAC4-UL130scramPNLIVForwardCACCGAGGCCAATAACCAGACTTACACCTTCTGCACACATCCCATCAATGTTCTCTGAGCCCGTCGCACGAGCAGaggatgacgacgataagtaggg
ReverseCGCGCGGTTTTCAAAATTCCCTGCTCGTGCGACGGGCTCAGAGAACATTGATGGGATGTGTGCAGAAGGTGTAAGcaaccaattaaccaattctgat
  • a Uppercase, HCMV homology; lowercase, homology to template plasmid pEP-Kan-S; bold, sequence of interest.