List of proteins identified in the immunoisolates of TAP- and GFP-tagged pUL83a

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  • a pUL83 was isolated via the protein A or GFP tag at 48 hpi and 72 hpi, respectively. Coisolated proteins were analyzed using MALDI MS and MS/MS analyses. The gi number, number of peptides detected, sequence coverage (%), XProteo score (d′) following database searching using the MS data, and peptide sequences confirmed by MS/MS are indicated for each identified protein. Only the proteins that were confirmed via the fragmentation of at least two peptides were included in the table. # indicates peptides unique to either IFI16 or IFI16b. The proteins classified as possible contaminants indicate proteins that we routinely observe as likely nonspecific associations in isolates from mammalian cells.