TABLE 1.

Kinetic and affinity constants for NP-RNA interactionsa

ProteinRNP activityMean ka (1/ms) ± SEMean Kd (1/s) ± SEMean KD (nM) ± max error
WTFully active6.00 × 105 ± 0.05 × 1059.48 × 10−3 ± 0.15 × 10−315.8 ± 0.4
I406SPartially active6.21 × 105 ± 0.07 × 1058.23 × 10−3 ± 0.16 × 10−313.3 ± 0.4
R422APartially active5.40 × 105 ± 0.07 × 1051.11 × 10−2 ± 0.02 × 10−220.6 ± 0.6
E449APartially active6.39 × 105 ± 0.08 × 1058.74 × 10−3 ± 0.19 × 10−313.7 ± 0.5
P410SFully active6.59 × 103 ± 0.09 × 1036.21 × 10−3 ± 0.09 × 10−3942 ± 27
P419SFully active7.49 × 103 ± 0.08 × 1032.83 × 10−3 ± 0.07 × 10−3378 ± 13
R267APartially active1.09 × 104 ± 0.02 × 1048.13 × 10−3 ± 0.10 × 10−3745 ± 23
E339AInactive3.40 × 103 ± 0.06 × 1032.92 × 10−3 ± 0.09 × 10−3858 ± 42
R416AInactive3.63 × 103 ± 0.04 × 1033.54 × 10−3 ± 0.06 × 10−3975 ± 27
V408S P410SInactive2.03 × 103 ± 0.04 × 1031.20 × 10−3 ± 0.09 × 10−3593 ± 56
L418S P419SInactive8.25 × 103 ± 0.07 × 1033.31 × 10−3 ± 0.05 × 10−3401 ± 9
  • a KD = kd/ka, where KD is affinity, kd is the dissociation rate, and ka is the association rate. Errors of ka and kd represent standard errors; errors of KD represent the maximum errors from each experiment.