TABLE 1.

TZMbl neutralization data of gp41-adsorbed IgG

Patient and sampleaNeutralizing activity (IC50 [μg/ml])b
Tier 1 virus (clade)Tier 2 virus (clade)
MW965.26 (C)BaL.26 (B)SS1196.1 (B)SF162.LS (B)DJ263.8 (A)6535.3 (B)RHPA4259.7 (B)TRO.11 (B)
Patient 1
    IgG14.149.0157.29.6128.8114.8308.8198.6
    gp41neg7.122.3108.54.2117.367.1148.264.8
    gp41pos11.5>55>55>55>55NTNT>55
Patient 2
    IgG2.020.392.96.0132.5203.6388.61.138
    gp41neg1.210.269.42.567.8273.5252.1>1.005
    gp41pos16.2>35>35>35*>35NTNT>35
Patient 3
    IgG10.127.2140.124.025.4489.957.4106.5
    gp41neg9.721.4105.122.427.4963.577.392.7
    gp41pos23.9>50>50>50>50NTNT>43
  • a IgG refers to the neutralizing activity in patients' total IgG fraction, whereas gp41neg refers to the activity in the gp41-depleted fraction, and gp41pos refers to the activity in the eluate of anti-gp41 antibodies after gp41 binding.

  • b Values in boldface represent the concentrations at which the tested serum samples showed 50% neutralization. *, values that almost reached the IC50 at the highest concentration tested; NT, not tested.