TABLE 2.

Mutations detected at a frequency of >1 within at least one sample

Mutation (amino acid substitution)No. of animalsSample (dog or virus) with this mutation (day)aMotif locationbMutation seen in epidemiological-scale equine H3N8Amino acid variable in equine H3N8?
A19T18271 (day 4)***NoNo (A)
S30N1Challenge virus**, 7151 (day 4)**NoYes (S, T)
D31G0Challenge virus**NoYes (D, N)
Y56H18271 (day 2)**, 8271 (day 4)*Near Ag CaNoNo (Y)
S83N28271 (day 2)*, 8271 (day 3)*, 8271 (day 4)**, 7151 (day 2)*, 7151 (day 3)****Ag EbYesYes (N, S, K)
S92N28271 (day 4)*, 7151 (day 4)***YesYes (S, N)
V118M17151 (day 2)****, 7151 (day 4)**************Near Ag ANoNo (L)
T126A3Challenge virus*, 8271 (day 4)*, 7151 (day 4)*, A1100**Ag BYesYes (T, A, I)
V130V17151 (day 3)*, 7151 (day 4)***Near Ag ANo
R135I16685 (day 2)******************************************* **********Ag ANoYes (R, G, T, E, S)
K156E2Challenge virus**, 8271 (day 3)*, 7151 (day 3)****Ag BYesYes (K, E, Q, N)
P185P17151 (day 4)***Near Ag BNoNo (P)
G218E4Challenge virus******, 8271 (day 2)*******, 7151 (day 2)******, 7151 (day 4)*************************, 5981 (day 2)****, 6685 (day 2)**Ag DbNoNo (G)
R220S4Challenge virus**********, 8271 (day 2)****************, 8271 (day 3)****, 8271 (day 4)*********, 7151 (day 2)***********, 7151 (day 3)********, 7151 (day 4)*, 5981 (day 2)******************************, 5981 (day 3)******************************, 6685 (day 2)**Ag DbNoNo (R)
P221P27151 (day 4)***, 6685 (day 2)*NoNo (P)
N248T0Challenge virus*****Near Ag DcNoYes (N, K)
P254P27151 (day 4)***, 6685 (day 2)*NoNo (P)
S270S0Challenge virus**NoNo (S)
  • a The number of sequences detected in each sample is indicated by the number of asterisks. The sampling day postinoculation is shown in parentheses.

  • b Ag, antigenic site.