TABLE 1.

Km values of typical synthetic peptide substrates and Ki values of typical inhibitors for recombinant soluble hMSPL and hTMPRSS13

Substrate or inhibitorKm (μM) or Ki (nM) for:
hMSPLhTMPRSS13
Substrates
    Boc-Q-A-R-MCA982.01789.0
    Boc-Q-R-R-MCA25.013.7
    Boc-L-K-R-MCA130.0230.0
    Boc-E-K-K-MCA890.0910.0
    Boc-R-V-R-R-MCA10.08.6
    Pyr-R-T-K-R-MCA8.77.4
    Pyr-K-K-K-R-MCA7.56.0
    Pyr-R-K-K-R-MCA12.07.1
Inhibitors
    Aprotinin10.516.3
    BBI19.231.7
    Dec-R-V-K-R-cmk2.90.9
    α1-PDXNINI
  • Km values of typical synthetic substrates derived from monobasic and multibasic substrates for hMSPL and hTMPRSS13 were measured as described previously (31). Ki values for Boc-Q-R-R-MCA, one of the efficient substrates, were measured. Pyr, pyroglutamyl; NI, no inhibition.