TABLE 2.

Antibodies used in this study

AntibodyTypeCloneHTUa bufferDilutioncSource
Anti-VZV IE62Rabbit pAbbNACitrate1:500 (IFp, IFc)PR Kinchington
Anti-VZV ORF4Rabbit pAbNACitrate1:500 (IFp, IFc)PR Kinchington
Anti-VZV ORF29Rabbit pAbNACitrate1:500 (IFp, IFc)PR Kinchington
Anti-VZV gEMouse IgG2bNACitrate1:10 (IFp), 1:100 (IHC, IFc)Chemicon International
Anti-human CD1aMouse IgG1MTB1Citrate1:10 (IHC)NovoCastra
Anti-human langerinMouse IgG2b12D6Citrate1:100 (IHC, IFp)NovoCastra
Anti-human CD83Mouse IgG2bHB15aCitrate1:10 (IHC)Immunotech
Anti-human DC-LAMPMouse IgG1104.G4Citrate1:5 (pdIHC)Immunotech
Anti-human DC-SIGN-PEMouse IgG2b120507Citrate1:500 (IHC)R&D Systems
Anti-human CD123Mouse IgG19F5EDTA1:10 (pdIHC)BD Biosciences
Anti-human DLEC (BDCA-2)Goat pAb IgGNACitrate1:10 (IFp), 1:50 (IFc)R&D Systems
Anti-human CD83-FITCMouse IgG1HB15eNA1:20 (FC)BD Biosciences
Anti-human DC-SIGN-FITCMouse IgG2bDCN46NA1:20 (FC)BD Biosciences
Anti-human Lin-1-FITCMouse MAb mixNANA1:20 (FC)BD Biosciences
Anti-human CD123-PEMouse IgG19F5NA1:20 (FC)BD Biosciences
Anti-human langerin-PEMouse IgG1DCGM4NA1:20 (FC)Coulter Immunotech
Anti-human HLA-DR-PerCPMouse IgG2aL243NA1:20 (FC)BD Biosciences
Anti-human CD11c-APCMouse IgG2bS-HCL-3NA1:20 (FC)BD Biosciences
Anti-human CD123-APCMouse IgG2aAC145NA1:20 (FC)Miltenyi Biotec
Anti-human MR-APCMouse IgG119.2NA1:20 (FC)BD Biosciences
Anti-human CD1a-PE-Cy5Mouse IgG1HI149NA1:20 (FC)BD Biosciences
Mouse IgG1 isotype controlMouse IgG1NANANABD Pharmingen
Mouse IgG2a isotype controlMouse IgG2aNANANABD Pharmingen
Mouse IgG2b isotype controlMouse IgG2bNANANABD Pharmingen
Whole rabbit serumNANANANASigma
Goat IgG isotype controlGoat pAbNANANAR&D Systems
  • a HTU, high-temperature unmasking.

  • b pAb, polyclonal antibody.

  • c IFp, immunofluorescent staining on paraffin sections; IFc, immunofluorescent staining on cell spots; IHC, immunohistochemistry; pdIHC, immunohistochemistry with polymer detection; FC, flow cytometry.