TABLE 2.

Virus loads in single-cell suspensions and resting CD4+ T cellsa

Monkey (treatment)TissueSingle-cell suspensiondResting CD4+ T cellsd
% CD4+ cellsb% HLA-DR+ cellscDNA levelRNA level% CD4+ cellsb% HLA-DR+ cellscDNA levelRNA level
Mmu 35339 (HAART)PBMC30.212.478<190.50.9292<2
MLN6014.647<197.50.12340<1
Spleen30.926.331<190.41.391<1
Jejunum36.32.8**92.21.1*<4
Mmu 35342 (HAART)PBMC45.64.38615093.60.59170<1
MLN50.816.490<196.70.323040
Spleen30.714.992<190.60.78**
Jejunum18.54<2<184.1ND53*
Mmu 35343 (HAART)PBMC53.47.271<293.80.471604
MLN58.423.5130<297.60.2623012
Spleen3536.595<2960.281904
JejunumNDND**79.60.2275014
Mmu 35349 (HAART)PBMC47.610.5180<292.41.12170<1
MLN37.830.42301094.40.37460<2
Spleen26.237.187<294.90.065470*
Jejunum16.44.25100*81.40.24490*
Mmu 35389 (HAART)PBMC41.913.7200<492.52.87910
MLN41.923.982<191.51170<3
Spleen28.443.634<1894.9310<5
Jejunum35.74.3**92.31.3529<3
Mmu 35483 (no HAART)PBMCNDND3701040NDND470390
MLNNDND375390NDND550870
SpleenNDND2901000NDND24009000
JejunumNDND**NDND**
  • a All tissue values are the means for two different tissue samples. All VL values are normalized to 2 × 106 copies of cellular IL-2 DNA measured in each sample.

  • b Percentage of CD3+ cells expressing CD4 and negative for CD8.

  • c Percentage of lymphocytes expressing HLA-DR.

  • d *, no sample or low nucleic acid level in sample; ND, not determined.