Statistic type | Result |
---|---|

Data collection | |

Model | VP35 IID K319A/R322A |

Space group | P2_{1}2_{1}2_{1} |

Cell dimension | a = 51.43, b = 66.07, c = 72.64 |

α = β = γ = 90 | |

Energy (eV)/wavelength (Å) | 12,666/0.979 |

Resolution (Å)^{c} | 36.32-1.70 (1.76-1.70) |

R_{merge}^{a} (%) | 8.9 (58.5) |

I/σ (I) | 9.4 (2.8) |

Completeness (%) | 97.5 (88.3) |

Redundancy | 6.34 (4.96) |

Refinement | |

Resolution (Å) | 36.32-1.70 |

No. of unique reflections | 27,195 |

R_{work}/R_{merge}^{b} | 19.1/24.6 |

No. of atoms | 2,169 |

No. of solvent sites | 251 |

B-factors, protein (Å^{2}) | 11.42 |

B-factors, water (Å^{2}) | 29.22 |

RMS bond lengths (Å) | 0.021 |

RMS bond angles (°) | 1.72 |

↵

*a**R*_{merge}= Σ_{i}Σ_{j}‖*I*_{ij}− <*I*_{j}> ‖/Σ_{i}Σ_{j}/_{ij}, where*i*runs over multiple observations of the same intensity and*j*runs over all crystallographically unique intensities.↵

*b**R*_{work}= Σ ‖‖*F*_{obs}‖ − ‖*F*_{calc}‖‖/Σ ‖*F*_{obs}‖, where ‖*F*_{obs}‖ > 0.*R*_{merge}was based upon 10% of the data randomly selected and not used in the refinement.↵

*c*Highest-resolution shell is shown in parentheses.